河南省及周边地区猪圆环病毒2型分子流行病学调查

为了解河南及周边地区猪圆环病毒2型(PCV2)的分子流行病学情况,运用PCR方法对2014—2015年采集到的河南及周边地区山东、山西、河北、甘肃5省共158份病料进行PCV2检测,将PCR扩增得到的PCV2 ORF2基因片段进行测序,分析研究PCV2遗传变异情况。结果显示,158份样品中,有69份样品为PCV2阳性,得到19株病毒的ORF2基因序列;将检测到的PCV2 ORF2序列与国内外分离株进行比对发现,核苷酸序列的同源性为89.9%~99.9%,其氨基酸序列同源性为88.0%~99.6%。综上可知,河南及周边地区PCV2流行广泛,病毒变异频繁。

Molecular Epidemiological Investigation of Porcine Circovirus Type 2 in Henan and Nearby Provinces

In order to investigate molecular epidemiology of porcine circovirus type 2 (PCV2),a total of 158 samples including serum samples,lungs,lymph node were collected between 2014 and 2015 in Henan,Hebei,Shandong,Shanxi and Gansu provinces.All the 158 samples were subjected to PCR and the positive samples were sequenced to obtain the whole ORF2 gene.The results showed that 69 samples were PCV2 positive according to PCR,and 19 ORF2 genes were acquired.Sequence comparision showed that the nucleotide sequence homologies of ORF2 genes were up to 89.9％-99.9％,and the amino acid homologies were 88.0％-99.6％.The results indicated that the PCV2 was endemic in Henan province and provinces nearby,and PCV2 varied frequently.