槟榔内生细菌BLG1的抗菌活性及其对水稻纹枯病的防效

为获得具有生防潜力的内生细菌,采用常规组织表面消毒法对槟榔进行内生细菌分离,共获得16株内生细菌。经筛选发现,菌株BLG1抑菌谱较广,尤其对水稻纹枯病菌的皿内抑菌率可达95.00%。进一步研究该菌株胞内及胞外粗提物的抑菌活性发现,该菌株活性物质主要分布在胞外正丁醇相中。盆栽试验表明,该菌株发酵液对水稻纹枯病有很好的防效,可达67.05%,与对照药剂10%井冈霉素水剂效果(66.47%)相当。因此,BLG1菌株具有深入研究和开发的价值。

Determination of Antimicrobial Activity and Control Effect on Rhizoctonia solani of Endophytic Bacterium BLG1 from Areca catechu L.

In order to obtain bio-control endophytic bacteria, sixteen endophytic bacteria were isolated from Areca catechu L. by conventional surface disinfection method. The strain BLG1 showed excellent antimicrobial activities,especially,the inhibition rate against Rhizoctonia solani reached 95. 00%. Crude extracts from endo- and extra-cell of the strain were tested, and the result showed that the active components mainly were distributed in extracellular part extracted by n-butyl alcohol. Pot experiment test showed that the strain BLG1 had good control effect (67. 05%) against Rhizoctonia solani,which was the same as 10% Jinggangmycin aqueous solution (66. 47%). Therefore,the strain BLG1 has values of further research and development.