紫薇茎段离体培养体系的优化

以矮首领紫薇当年生半木质化带腋芽茎段、硬枝茎段以及叶片为外植体,从消毒时间、启动培养基、增殖培养基和生根培养基选择等方面进行离体培养体系的优化。结果表明,茎段为较好的外植体。最佳消毒时间:带腋芽茎段用0.1%升汞处理8 min,诱导率为66.67%;带水培芽茎段用0.1%升汞处理4 min,诱导率为65.55%;叶片用0.1%升汞处理4 min,诱导率为55.56%。带腋芽茎段最佳启动培养基为MS+6-BA 1.0 mg/L+NAA 0.10 mg/L,诱导率为83.33%。腋芽和水培芽的最佳增殖培养基均为MS+6-BA 2.0 mg/L+NAA 0.30 mg/L。腋芽增殖系数为2.42,芽长为3.2 cm;水培芽增殖系数为3.21,芽长为2.1 cm。腋芽最佳生根基本培养基为1/2MS,最佳生根培养基为1/2MS+IBA 0.5 mg/L,生根率为96.67%;水培芽最佳生根培养基为1/2MS+IBA0.5 mg/L+活性炭(AC)0.1 g/L,生根率为81.25%。

In Vitro Culture System Optimization of Lagerstroemia indica Stems

One-year-old half-lignified stems with axillary buds,hardwood stems with hydroponic buds and leaves of Lagerstroemia indica 'Aishouling' were used as explants for in vitro culture system optimization,including sterilization time,induction medium,proliferation medium and basic rooting medium.The results showed that the stems were the optimal explants.The optimal sterilization time of 0.1% HgCl2:stems with axillary buds was treated for 8 min,and the induced rate was 66.67%;stems with hydroponic buds was treated for 4 min,and the induced rate was 65.55%;leaves was treated for 4 min,and the induced rate was 55.56%.By taking the stems as explants,the best induction medium of stems with axillary buds was MS+6-BA 1.0 mg/L+NAA 0.10 mg/L,with the induction rate of 83.33%.The optimal proliferation media for axillary buds and hydroponic buds were both MS+6-BA 2.0 mg/L+NAA 0.30 mg/L.The proliferation coefficient of axillary buds was 2.42,and the shoot length was 3.2 cm;the proliferation coefficient of hydroponic buds was 3.21,and the shoot length was 2.1 cm.The best basic rooting medium for axillary buds was 1/2MS;the best rooting medium was 1/2MS+IBA 0.5 mg/L,and the rooting rate was 96.67%.The best rooting medium for hydroponic buds was 1/2MS+IBA 0.5 mg/L+AC 0.1 g/L,and the rooting rate was 81.25%.