牦牛EPO基因的PCR-SSCP多态性研究

以巴州牦牛、大通牦牛和九龙牦牛为对象,对促红细胞生成素(EPO)基因用PCR-SSCP方法进行了多态性检测。结果表明:大通牦牛、巴州牦牛、九龙牦牛具有较丰富的遗传多样性,表现为AA,AB和BB3种基因型,AB为优势基因型,A为优势等位基因;经χ2适合性检验,3个牦牛品种在该基因位点上均处于Hardy-Weinberg平衡状态。同时发现随着海拔高度的增加,A等位基因频率逐渐升高,BB型的频率则降低,群体杂合度和多态信息含量也逐渐降低,这种变化可能与牦牛对低氧的适应能力有一定的关系。

Study of Polymorphism on EPO Gene in Yak by PCR-SSCP

In this paper,the polymorphisms of hemopoietin(EPO)gene in Bazhou yak,Datong yak and Jiulong yak were investigated by PCR-SSCP and the results showed that the genetic diversity is rich among the three yak breeds.The genotypes are AA,AB and BB,in which AB genotype is the dominant genotype and allele A is the dominate allele.The χ2 compatibility test indicates that the gene frequencies of all of the three yak breeds are in Hardy-Weinberg equilibrium state.Simultaneously,this research also exhibits that the gene frequency of allele A rises up accompany with the increase of the altitude,while the BB allele frequency,colony heterozygosity and polymorphic information content rise down.This change possibly relates to the adaptive capacity to the oxygen-poor environments.