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| moFcγRⅢ、γ链真核表达载体的构建及其共转染稳定细胞系的建立 | |
| 引用本文： | 席俊,唐志鹏,张利娜,宋爱宾,王少兵,张改平.moFcγRⅢ、γ链真核表达载体的构建及其共转染稳定细胞系的建立[J].河南农业科学,2011,40(8):190-193. |
| 作者姓名： | 席俊 唐志鹏 张利娜 宋爱宾 王少兵 张改平 |
| 作者单位： | 1. 河南工业大学粮油食品学院,河南郑州,450052 2. 福山区畜牧局,山东福山,265500 3. 招远市畜牧局,山东招远,265400 4. 河南省农业科学院农业部动物免疫学重点开放实验室,河南省动物免疫学重点实验室,河南郑州450002 |
| 基金项目： | 河南工业大学引进人才专项(2010BS010) |
| 摘 要： | 将moFeγRⅢ、γ链编码区eDNA分别亚克隆到真核表达栽体pcDNA3的巨细胞病毒启动子下游,构建了重组表达质粒pe3moRⅢ、pe3moγ;用PvuⅠ线性化重组质粒,以线性化重组质粒其转染COS -7细胞,用玫瑰花环试验检测moFeγRⅢ在转染细胞表面的表达,通过G418抗性筛选和单细胞克隆,在转染细胞表面稳定表达... |
| 关 键 词： | moFcγRⅢ COS -7细胞 玫瑰花环试验 真核表达 共转染 |
Establishment of a Cell Line Cotransfected with a Eukaryotic Expression Vector Stably Expressing moFcγRⅢ and γ Chain |
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| XI Jun,TANG Zhi-peng,ZHANG Li-na,SONG Ai-bin,WANG Shao-bing,ZHANG Gai-ping.Establishment of a Cell Line Cotransfected with a Eukaryotic Expression Vector Stably Expressing moFcγRⅢ and γ Chain[J].Journal of Henan Agricultural Sciences,2011,40(8):190-193. | |
| Authors: | XI Jun TANG Zhi-peng ZHANG Li-na SONG Ai-bin WANG Shao-bing ZHANG Gai-ping |
| Institution: | XI Jun1,TANG Zhi-peng2,ZHANG Li-na3,SONG Ai-bin3,WANG Shao-bing3,ZHANG Gai-ping4(1.School of Food Science and Technology,Henan University of Technology,Zhengzhou 450052,China,2.Fushan Bureau of Animal Husbandry,Fushan 265500,3.Zhaoyuan Bureau of Animal Husbandry,Zhaoyuan 265400,4.Key Laboratory for Animal Immunology of the Ministry of Agriculture/Henan Provincial Key Laboratory ofAnimal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
| Abstract: | The cDNA for the complete encoding region of moFcγRⅢ and γ-chain were separately cloned into the mammalian expression vector pcDNA3 to construct expressing plasmids,pc3moRⅢand pc3moγ.COS-7 cells were cotransfected with the linear expressing plasmids,selected by G418 and detected by rosette assay for the expression of moFcγRⅢ.The establishment of the stably-transfected cell line expressing target gene provides a basis for further studies on the function of the moFcγRⅢ gene. |
| Keywords: | moFcγRⅢ COS-7 cells Rosette assay Eukaryotic expressio Coexpression |
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