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| 华南虎绦虫ITS及5.8S rDNA序列扩增与序列分析 | |
| 引用本文： | 张新高,侯杰,陈武,程田,林瑞庆.华南虎绦虫ITS及5.8S rDNA序列扩增与序列分析[J].河南农业科学,2011,40(8):205-209. |
| 作者姓名： | 张新高 侯杰 陈武 程田 林瑞庆 |
| 作者单位： | 1. 深圳市南山区动物防疫监督所,广东深圳,518051 2. 华南农业大学兽医学院,广东广州,510642 3. 广州动物园,广东广州,510070 |
| 基金项目： | 教育部“长江学者和创新团队发展计划”创新团队项目(IRT0723) |
| 摘 要： | 以从广州动物园华南虎体内采集的2条绦虫作为研究对象,利用核糖体DNA(rDNA)内转录间隔区序列(ITS)的遗传标记特点,用保守引物BD1和BD2对提取的DNA进行PCR扩增,经胶回收纯化后,连接到pGEM-T Easy载体上,然后转化并鉴定出阳性菌落,对菌落进行PCR扩增并测序,将测序结果与GenBank公布的相关绦虫ITS序列进行对比分析,结果显示,采自广州动物园华南虎的2条绦虫ITS及5.8S rDNA序列总长均为1387bp,序列相似性为100%,与猬迭宫绦虫(Spirometra erinaceieuropaei)、舌形属绦虫(Diphyllobothrium ligula)、宽节双叶槽绦虫(Diphyllobothriumlatum)的ITS及5.8S rDNA序列相似性分别为96.7%、66.5%、71.5%,结果显示,此次分离的绦虫属于迭宫属的猬迭宫绦虫,所测得的华南虎猬迭宫绦虫的ITS序列为首次报道。 |
| 关 键 词： | 华南虎 绦虫 核糖体DNA 内转录间隔区序列 鉴定 |
Amplification and Sequence Analysis of the ITS and 5.8S rDNA of Cestodes From Panthera tigris amoyensis in China |
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| ZHANG Xin-gao,HOU Jie,CHEN Wu,CHENG Tian,LIN Rui-qing.Amplification and Sequence Analysis of the ITS and 5.8S rDNA of Cestodes From Panthera tigris amoyensis in China[J].Journal of Henan Agricultural Sciences,2011,40(8):205-209. | |
| Authors: | ZHANG Xin-gao HOU Jie CHEN Wu CHENG Tian LIN Rui-qing |
| Institution: | ZHANG Xin-gao1,HOU Jie2,CHEN Wu3,CHENG Tian2,LIN Rui-qing2(1.Nanshan District Institute of Animal Diseases Prevention and Control,Shenzhen 518051,China,2.College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,3.Guangzhou Zoo,Guangzhou 510070,China) |
| Abstract: | Utilizing the first and second internal transcribed spacers of ribosomal DNA(ITS rDNA) as the genetic marker,the specific identity of the cestodes from a South China tiger(Panthera tigris amoyensis) in Guangzhou Zoo was performed in the present study.The ITS rDNA was amplified by PCR using the conserved primer pair BD1 and BD2.The PCR products were purified,cloned into pGEM-T Easy vector,and then the positive clones were selected for sequencing.DNA sequences were compared with relevant sequences previously ... |
| Keywords: | Panthera tigris amoyensis Cestodes Ribosomal DNA(rDNA) Internal transcribed spacers(ITS) Identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |