玉米自交系幼胚培养与基因枪转化研究

玉米自交系幼胚在添加 1～ 2mg/L 2 ,4-D的N6培养基上能够诱导形成不同类型的胚性愈伤组织。愈伤组织的诱导率与基因型和 2 ,4-D浓度有关 ,6个自交系的诱导率在 5 2 %～ 89%。将淡黄色颗粒状结构致密型愈伤组织在N6诱导培养基上继代培养 2 0个月 ,仍具有较强的分化能力。在附加 2mg/L 6 -BA和0 .2mg/LNAA的N6分化培养基上 ,有 2 7%～ 41%的愈伤组织分化成苗。通过基因枪轰击成功地将 pbarGUS基因转入玉米细胞 ,获得了大量的转化胚性愈伤组织。用 0 .4mol/L的甘露醇高渗处理幼胚 ,能显著提高pbarGUS基因在细胞中的表达 ,高渗处理转化率较对照提高了 3倍

Study on Maize Inbred Immature Embryo Culture and Transformation by Particle Bombardments

Study on factors in fluencing maize immature embryo culture and pbar GUS gene transformation by particle bombardments have been studied.Showed that genetypes and 2,4-D had some effect on embryonic callus induction.Adding 2 mg/L 2,4-D to N\-6 medium was better in callus induction than adding 1 mg/L 2,4-D.The callus induction of 52%～89% had been obtained in 6 inbreds.A lot of embryonic callus could got by introducing pbar GUS gene into maize cell with particle bombardment.High osmotic treatment on immature embryo with 0.4mol/L mannitol could improve transient expression of pbar GUS gene,with the transformation rate increasing 3 times than ck.