| 苔藓植物SRAP反应体系的优化 |
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| 引用本文： | 张安世,邢智峰,韦慧彦,张为民,刘永英.苔藓植物SRAP反应体系的优化[J].河南农业科学,2009(11). |
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| 作者姓名： | 张安世 邢智峰 韦慧彦 张为民 刘永英 |
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| 作者单位： | 焦作师范高等专科学校,生物系,河南,焦作,454001 |
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| 基金项目： | 河南省科技攻关项目，河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 以黄灰藓总DNA为材料,对影响SRAP-PCR反应的模板DNA、Mg2+、dNTP、引物和TaqDNA聚合酶浓度等因素进行了优化,分析了各因素对SRAP-PCR扩增结果的影响。结果表明,在25μLSRAP-PCR反应体系中,最佳反应条件为:模板DNA40ng;Mg2+浓度2.0mmol/L;dNTP浓度0.2mmol/L;正反引物15pmol;TaqDNA聚合酶2.0U。在此条件下,引物组合Me5/em7对13种苔藓植物扩增的条带清晰、多态性好,表明此反应条件适合于苔藓植物的SRAP-PCR反应体系。
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| 关 键 词： | 苔藓 序列相关扩增多态性 优化 反应体系 |
Optimization of SRAP Reaction System in Bryophytes |
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| ZHANG An-shi,XING Zhi-feng,WEI Hui-yan,ZHANG Wei-min,LIU Yong-ying.Optimization of SRAP Reaction System in Bryophytes[J].Journal of Henan Agricultural Sciences,2009(11). |
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| Authors: | ZHANG An-shi XING Zhi-feng WEI Hui-yan ZHANG Wei-min LIU Yong-ying |
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| Abstract: | To obtain the optimum SRAP-PCR reaction system for Hypnum pallescens,5 factors(Template DNA,Mg2+,dNTP,primer,Taq DNA polymerase)influencing SRAP-PCR reaction system was tested and analysed their effects on SRAP-PCR amplification.Reaction in 25μL reaction system containing 40ng template DNA,2.0mmol/L MgCl2,0.2 mmol/L dNTPs,15 pmol primer and 2.0U Tag DNA polymerase got the optimal results.Under this optimal condition,clear and polymorphic bands from 13 species of bryophytes was obtained using primer Me5/em7.The results showed that the reaction condition was suitable for SRAP-PCR reaction system of bryophytes. |
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| Keywords: | Bryophytes SRAP Optimization Reaction system |
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