| 小玉竹组织培养及无性系的建立 |
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| 引用本文： | 张卓,姜巍,张巍巍,佟少明,姜长阳.小玉竹组织培养及无性系的建立[J].河南农业科学,2008(3):92-95. |
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| 作者姓名： | 张卓 姜巍 张巍巍 佟少明 姜长阳 |
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| 作者单位： | 辽宁师范大学,生命科学学院,辽宁,大连,116029 |
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| 基金项目： | 辽宁师范大学校科研和教改项目 |
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| 摘 要： | 以小玉竹根茎为外植体,以MS为基本培养基,附加不同浓度的6-BA,2,4-D,IAA,NAA,诱导根茎形成愈伤组织,并使愈伤组织分化形成不定芽,培养成生根试管苗,建立起小玉竹无性系。结果表明,MS+6-BA 0.4 mg/L+NAA 1.0 mg/L是根茎愈伤组织诱导的理想培养基;MS+6-BA 0.2 mg/L+NAA 0.2 mg/L是愈伤组织和不定芽分化培养的理想培养基;1/3MS+IAA 0.4 mg/L是试管苗生根培养的理想培养基。
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| 关 键 词： | 小玉竹 组织培养 愈伤组织 无性系 |
| 文章编号： | 1004-3268(2008)03-0092-04 |
| 修稿时间： | 2007年8月23日 |
Tissue Culture and Establishment of Asexual System for Polygonatum humile Fisch |
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| ZHANG Zhuo,JIANG Wei,ZHANG Wei-wei,TONG Shao-ming,JIANG Chang-yang.Tissue Culture and Establishment of Asexual System for Polygonatum humile Fisch[J].Journal of Henan Agricultural Sciences,2008(3):92-95. |
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| Authors: | ZHANG Zhuo JIANG Wei ZHANG Wei-wei TONG Shao-ming JIANG Chang-yang |
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| Abstract: | In the study,the stems and roots of Polygonatum humile Fisch were used as explants for tissue culture in MS medium with different concentrations of 6-BA,2,4-D,IAA,NAA to induce calluses,differentiate adventitious buds and roots,and then establish the asexual system of Polygonatum humile Fisch.The results indicated that the ideal medium to induce calluses was MS 6-BA0.4mg/L NAA1.0mg/L;The MS medium with 6-BA0.2mg/L and NAA0.2mg/L was suitable for formation of calluses and differentiation of adventitious buds;The medium of 1/3 MS IAA0.4mg/L was proper for rooting of tube seedlings. |
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| Keywords: | Polygonatum humile Fisch Culture of tissue Callus Asexual system |
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