| 温度及培养基中添加物对辣椒花药培养胚状体诱导的影响 |
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| 引用本文： | 刘广霞,张晓伟,蒋武生,原玉香,姚秋菊.温度及培养基中添加物对辣椒花药培养胚状体诱导的影响[J].河南农业科学,2009(5). |
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| 作者姓名： | 刘广霞 张晓伟 蒋武生 原玉香 姚秋菊 |
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| 作者单位： | 1. 河南农业大学园艺学院,河南,郑州,450002
2. 河南省农业科学院,园艺研究所,河南,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目 |
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| 摘 要： | 对11个辣椒基因型材料进行了花药培养研究,分别研究了温度处理、培养基中添加无机盐和激素对辣椒胚状体诱导的影响。结果表明:4℃低温预处理花蕾3~5 d和接种后33℃高温处理7~9 d均可大大提高胚状体诱导率;MS培养基中添加50μmol/L的AgNO3大幅度提高了胚状体诱导率。添加适量的激素也可以提高辣椒培养效果,其中,添加2,4-D或KT的最适浓度为0.1 mg/L,而添加较高浓度的NAA(1.0 mg/L)辣椒胚状体诱导率达到最高。
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| 关 键 词： | 辣椒 花药培养 胚状体 诱导率 |
Effects of Tempreture and Additives in Medium on Embryoid Induction in Anther Culture of Pepper(Capsicum annuum L.) |
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| LIU Guang-xia,ZHANG Xiao-wei,JIANG Wu-sheng,YUAN Yu-xiang,YAO Qiu-ju.Effects of Tempreture and Additives in Medium on Embryoid Induction in Anther Culture of Pepper(Capsicum annuum L.)[J].Journal of Henan Agricultural Sciences,2009(5). |
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| Authors: | LIU Guang-xia ZHANG Xiao-wei JIANG Wu-sheng YUAN Yu-xiang YAO Qiu-ju |
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| Institution: | 1.College of Horticulture;Henan Agricultural University;Zhengzhou 450002;China;2.Institute of Horculture;Henan Academy of Agricultural Sciences;China |
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| Abstract: | 11 genotypes of peppers were used in anther culture to study the factors affecting embryoid induction and formation.3-5d low-temperature pretreatment on flower buds or 7-9d high temperature treatment after inoculation could increase the rate of induction.50μmol/L silver nitrate benefits embryoid induction markedly.Adding suitable concentration of hormones in MS medium increased induction rate of embryoid,0.1mg/L KT,0.1mg/L 2,4-D or 1.0mg/L NAA was the optimum concentration for pepper anther culture. |
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| Keywords: | Pepper Anther culture Embryoid Induction rate |
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