| 花生幼叶丛生芽成苗高频再生体系的建立 |
| |
| 引用本文： | 苗利娟,黄冰艳,张新友,陈占宽,严玫,易明林.花生幼叶丛生芽成苗高频再生体系的建立[J].河南农业科学,2008(8). |
| |
| 作者姓名： | 苗利娟 黄冰艳 张新友 陈占宽 严玫 易明林 |
| |
| 作者单位： | 河南省农作物新品种重点实验室,河南,郑州,450002 |
| |
| 基金项目： | 国家转基因植物专项基金 |
| |
| 摘 要： | 以花生品种豫花14号4 d苗龄的幼叶诱导的丛生芽为研究对象,对丛生芽的伸长和植株再生进行了研究。分别将丛生芽继代到4种不同的培养基上促进芽伸长,其中,以继代培养基MS+6-BA 2 mg/L+NAA 0.4 mg/L+AgNO32 mg/L植株再生率最高。当丛生芽长至3 cm左右时,移入生根培养基诱导生根,获得完整的再生植株。
|
| 关 键 词： | 花生 丛生芽 组织培养 植株再生 |
Construction of Higher Efficient Peanut Regeneration System from the Growth of Leaflet |
| |
| MIAO Li-juan,HUANG Bing-yan,ZHANG Xin-you,CHEN Zhan-kuan,YAN Mei,YI Ming-lin.Construction of Higher Efficient Peanut Regeneration System from the Growth of Leaflet[J].Journal of Henan Agricultural Sciences,2008(8). |
| |
| Authors: | MIAO Li-juan HUANG Bing-yan ZHANG Xin-you CHEN Zhan-kuan YAN Mei YI Ming-lin |
| |
| Abstract: | Genetic engineering was an effective way to develop peanut variety.Plant regeneration were studied with the induced proliferation buds of 4d seedling leaflet of peanut variety Yuhua No.14.Proliferation buds were planted on 4 subculture.Result showed that subculture medium MS 6-BA 2mg/L NAA0.4mg/L AgNO3 2mg/L had the highest rate of plant regeneration.Regeneration plant could be get by transplanted the 3cm long proliferation buds into the rooting medium. |
| |
| Keywords: | Peanut Cluster shoots Tissue culture Regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
