大白菜核不育复等位基因的鉴定与分布

为摸清大白菜核不育复等位基因在3种生态型大白菜品系中的分布特性,利用基因型为MsMs的大白菜纯合显性雄性不育株为母本,与68个正常可育的直筒、卵圆和平头型大白菜可育品系杂交,统计后代育性分离比例,鉴定各品系基因型,计算不同生态型可育品系在核不育位点上的基因频率和基因型频率。结果表明,直筒型大白菜可育品系以纯合隐性基因型msms为主,占71.43%。可育基因ms频率为76.79%,恢复基因Msf频率为23.21%;卵圆型大白菜可育品系3种基因型MsfMsf,Msfms和msms分别占42.42%,39.40%和18.18%。Msf基因频率为62.12%,ms基因频率为37.88%;平头型大白菜可育品系以纯合隐性基因型msms为主,占57.14%。Msf基因频率为33.33%,ms基因频率为66.67%。直筒型和平头型大白菜可育品系的核不育复等位基因位点上以ms基因为主,而卵圆型大白菜可育品系的核不育复等位基因位点上以Msf基因为主。

Identification and Distribution of Male Sterile Multiple Alleles in Chinese Cabbage

The objective of the paper was to characterize the distribution of nuclear sterile multiple alleles in 3 ecotypes of Chinese cabbage lines.A dominant male sterile Chinese cabbage MsMs was used as the test cross parent to make hybridizations with 68 male fertile cabbage lines,which belong to Straighttubbish,Ovate or Level-head ecotypes,respectively.By calculating the fertility segregation ratio of the progenies,and identifying the genotype of each male fertile lines,the gene and genotype frequency of the nuclear sterile multiple allelic loci were estimated in the three ecotypes.The results showed that the purified recessive genotype msms was superior to others in Straight-tubbish ecotype,accounting for 71.43% of the total genotype;The frequency of the male fertile gene ms and restore gene Msf accounted for 76.79% and 23.21%,respectively,in Straight-tubbish ecotype.The MsfMsf,Msfms and msms accounted for 42.42%,39.40% and 18.18%,respectively,in Ovate ecotype;The frequency of the restore gene Msf and fertile gene ms accounted for 62.12% and 37.88%,respectively,in Ovate-cabbage ecotype.The purified recessive genotype msms was superior to others in level-head ecotype,accounting for 57.14% of the total genotype;The frequency of restore gene Msf and fertile gene ms were separately accounting for 33.33 % and 66.67% in Level-head ecotype.