| 大花蕙兰高频再生体系的建立 |
| |
| 引用本文： | 梁新安,杨录军,王慧瑜,孙新政.大花蕙兰高频再生体系的建立[J].河南农业科学,2006(10):85-87. |
| |
| 作者姓名： | 梁新安 杨录军 王慧瑜 孙新政 |
| |
| 作者单位： | 1. 河南农业职业学院,河南,中牟,451450
2. 郑州市农林科学研究所,河南,郑州,450005 |
| |
| 摘 要： | 以大花蕙兰的不同部位作外植体,对其启动诱导;采用正交试验设计等方法筛选大花蕙兰类原球茎诱导、增殖与生根壮苗的最佳培养基。结果表明:在大花蕙兰离体再生过程中,侧芽是最容易启动的外植体;MS 6-BA 1.0 mg/L NAA 0.1 mg/L AC 0.1 g/L是类原球茎诱导的最佳培养基;1/2MS 6-BA 1.0 mg/L NAA 0.1 mg/L是类原球茎增殖和幼苗分化的最适宜培养基;芽分化后,在1/2MS NAA 1.0 mg/L GA31.0 mg/L 香蕉泥150 g/L培养基中生根壮苗效果最好。
|
| 关 键 词： | 大花蕙兰 类原球茎 再生植株 |
| 文章编号： | 1004-3268(2006)10-0085-03 |
| 收稿时间： | 2006-06-23 |
| 修稿时间： | 2006年6月23日 |
Establishment of High-frequency Regeneration System for Cymbidium |
| |
| LIANG Xin-an,YANG Lu-jun,WANG Hui-yu,SUN Xin-zheng.Establishment of High-frequency Regeneration System for Cymbidium[J].Journal of Henan Agricultural Sciences,2006(10):85-87. |
| |
| Authors: | LIANG Xin-an YANG Lu-jun WANG Hui-yu SUN Xin-zheng |
| |
| Institution: | 1. Henan Agriculture Vocational College, Zhongmu 451450, China; 2. Zhengzhou Institute of Agriculture and Forestry Science,Zhengzhou 450005, China |
| |
| Abstract: | The different parts of cymbidium were used as the explants to select the optimal media for the induction,multiplying and the rooting and sprouting of protocorm-like-body of by the orthogonal design.The results showed as follows: the axially bud was more easily started as explants.MS medium supplemented with 6BA 1.0 mg/L and NAA 0.1 mg/L and AC 0.1 g/L was optimal for the induction of protocorm-like-body.The best medium for the propagation of protocorm-like-body and differentiation of shoots was 1/2MS 6BA 1.0 mg/L NAA 0.1 mg/L.Optimal medium for the rooting and strong sprout was 1/2MS NAA 1.0 mg/L GA_3 1.0 mg/L banana mud 150 g/L. |
| |
| Keywords: | Cymbidium Protocorm like-body Regenerated plantlet |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
