| 拟南芥活性氧不敏感型突变体的筛选方法建立 |
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| 引用本文： | 何金环,李存法,宋纯鹏.拟南芥活性氧不敏感型突变体的筛选方法建立[J].河南农业科学,2004(6):30-34. |
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| 作者姓名： | 何金环 李存法 宋纯鹏 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校生物工程系,河南,郑州450011
2. 河南大学生命科学学院 |
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| 基金项目： | 国家自然科学基金资助课题 (G1 9990 1 1 70 0 ) |
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| 摘 要： | 以模式植物拟南芥为材料 ,采用EMS化学诱变方法 ,在 1 0 - 4 mol/L的H2 O2 选择条件下 ,以幼苗根在重力作用下的弯曲生长为指标 ,对 5 0 0 0 0株拟南芥幼苗进行筛选 ,得到了 2 6株潜在拟南芥活性氧不敏感突变体。此突变体的获得为探索氧化信号分子的作用机制及其在信号转导网络中的作用提供了直接的材料。
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| 关 键 词： | 拟南芥 活性氧 根弯曲法 筛选 突变体 |
| 文章编号： | 1004-3268(2004)06-0030-05 |
| 修稿时间： | 2003年12月22 |
Establishment of Isolation Ways of Arabidopsis Mutants with Reactive Oxygen Species Insensitivity |
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| HE Jin-huan ,LI Cun-fa ,SONG Chun-peng.Establishment of Isolation Ways of Arabidopsis Mutants with Reactive Oxygen Species Insensitivity[J].Journal of Henan Agricultural Sciences,2004(6):30-34. |
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| Authors: | HE Jin-huan LI Cun-fa SONG Chun-peng |
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| Institution: | HE Jin-huan 1,LI Cun-fa 1,SONG Chun-peng 2 |
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| Abstract: | ethylmethane sulfonate (EMS)-mutagenized Arabidopsis M 2 seeds from reactive oxygen insensitive mutants were screened,and 26 putative reactive oxygen insensitive mutants were obtained using root-bending assay on 10 -4 mol/L H 2O 2 agar plates.Undoubtedly,the acquisition of reactive oxygen species-insensitive mutants not only offered perfect materials for revealing the molecular mechanism of reactive oxygen species generation in plant cells under various environmental stress,but also provides new insights into oxidative signal transduction in plant cells. |
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| Keywords: | Arabidopsis thaliana Reactive oxygen species Root-bending assay Screening Mutant |
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