5株堆型艾美耳球虫顶质体rpoB基因的扩增及序列分析

以5株堆型艾美耳球虫为研究对象,对其顶质体rpoB基因的部分序列进行了PCR扩增、测序及序列分析,并与GenBank上发表的柔嫩艾美耳球虫及其他顶复门寄生虫相关序列进行比较,研究顶质体的遗传变异情况。结果表明,从堆型艾美耳球虫不同来源虫株均获得了469bp的目的片段,应用DNAStar软件进行序列对比发现,5株堆型艾美耳球虫的顶质体rpoB序列完全一致,与柔嫩艾美耳球虫相应序列的相似性为92.75%,而与刚地弓形虫和疟原虫的相似性分别为67.23%和64.62%。首次报道了堆型艾美耳球虫rpoB序列,显示rpoB基因序列保守,不同来源堆型艾美耳球虫虫株的序列没有差异,为进一步研究艾美耳球虫的群体遗传学奠定了基础。

PCR Amplification and Sequence Analysis of Apicoplast rpoB of Five Eimeria acervulina Strains

To research the genetic variation of apicoplast rpoB gene of Eimeria acervulina,the partial sequence of rpoB was amplified by polymerase chain reaction(PCR) and a 469 bp fragment was obtained from five strains of E.acervulina.Sequence comparison and comparative analysis of the rpoB gene sequences of Eimeria tenella with other reference apicomplexan parasites available in GenBank database showed that there was no nucleotide difference among the rpoB genes of five E.acervulina strains,and the sequence similarities were 92.75%,67.23%,and 64.62% compared with corresponding sequences of E.tenella,Toxoplasma gondii,and Plasmodium,respectively.The results revealed that the rpoB sequence was conserved in different strains of E.acervulina and provided a foundation for further studies of population genetics of Eimeria.