烟草细胞凋亡过程中核酸酶活性研究

以烟草为材料 ,以 2 -甲基萘醌为诱导物 ,研究了植物细胞凋亡过程中核酸酶活性 ,结果表明 :2 -甲基萘醌诱导的烟草原生质体使鸡血红细胞发生了典型的凋亡现象 ,加入Zn2 +、EDTA、EGTA、AC -DEVD -CHO的样品无“DNAladder”出现 ;加入Ca2 +、Mg2 +后 ,“DNAladder”重新形成。 2 -甲基萘醌诱导的胞浆提取物和细胞核与只加细胞色素C诱导的胞浆提取物在电泳图上出现相同的蛋白条带 ,分子量约 36KD。从而表明 :烟草细胞凋亡过程中有特异核酸酶被激活 ,该核酸酶活性依赖于Ca2 +、Mg2 +,被Zn2 +、AC -DEVD -CHO抑制 ,该酶活性与caspase凋亡通路有关

Studies on the Activity of Nuclease during the Apoptosis of Tobacco Cell

The studies with tobacco as tested material and 2-dimethyl naphthaquinone as inducer showed that the tobacco protoplasm induced by 2-dimethyl naphthaquinone made the erythrocyte of chicken occure typic apoptosis.No “DNA ladder”appearsed if Zn 2+,EDTA,EGTA,AC-DEVD-CHO were added in samples.“DNA ladder”reformsed if Ca 2+,Mg 2+ were added.The cytoplasm extract and karyon induced by 2-dimethyl naphthaquinone and cytoplasm extract induced by cytochrome C,showed the similar protein bands on the electrophoresis picture,with molecular weight of about 36KD.It is concluded that during the decline of tobacco cell,the nuclease is actived,and its activity depends on Ca 2+, Mg 2+ and is restrained by Zn 2+,AC-DEVD-CHO. The enzyme exists in cytoplasm and karyon at the same time.