Ar~+介导玉米DNA水稻不同pH条件下蛋白水解酶的表达

利用离子束介导技术,对水稻豫粳6号种子进行Ar+照射处理,然后用玉米基因组DNA介导液进行转化处理。采用复性电泳技术对其幼苗叶片在不同pH下的蛋白水解酶瞬时表达情况进行了分析。结果表明,不同pH条件下各处理蛋白水解酶表达存在明显差异。与对照相比,Ar+介导玉米DNA的水稻幼苗叶片在pH 4.5条件下检测到1条129 kD的新酶带,缺失41、97、192 kD的3条酶带;在pH 7.0时检测到1条167 kD的新酶带,缺失1条67 kD的酶带;在pH 8.5条件下缺失1条61 kD的酶带,并且在192~234 kD检测到1条活性较强的亮带区。这表明,Ar+介导玉米DNA的水稻在蛋白质表达水平上产生显著差异,这可能是玉米DNA片段进入受体水稻细胞并引起受体基因表达改变的结果。

Analysis of Proteinases in Rice with Maize DNA Mediated by Ar Ion Beam

The seeds of rice Yujing 6 were irradiated by Ar ion beam,and then were transferred with maize genome DNA.The proteinases of rice leaves were analysed by renaturation electrophroresis in different pH.The results showed that the expression of proteinases had changed obviously.Compared to the control,a new proteinase(129kD) was present and three proteinases(41,97 and 192kD) were Absent in condition of pH 4.5,a new proteinase(167kD) present and one(67kD) Absent in condition of pH 7.0,while a new proteinase(61kD) Absent in condition of pH8.5.Besides,a proteinase with strong activity covered the area of 192kD-234kD molecular weight was detected.The obvious diversity of proteinase expression indicated that the maize DNA may have been transferred into the rice,and changed the gene expression of rice.