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| 抗鸡传染性法氏囊病病毒单克隆抗体识别的抗原表位分析 | |
| 引用本文： | 肖治军,张改平,杨汉春,杨艳艳,李学伍,邓瑞广,赵东.抗鸡传染性法氏囊病病毒单克隆抗体识别的抗原表位分析[J].河南农业科学,2006(11):105-109. |
| 作者姓名： | 肖治军 张改平 杨汉春 杨艳艳 李学伍 邓瑞广 赵东 |
| 作者单位： | 1. 中国农业大学动物医学院,北京,100094;河南省农业科学院生物技术研究所,河南,郑州,450002 2. 河南省农业科学院生物技术研究所,河南,郑州,450002 3. 中国农业大学动物医学院,北京,100094 |
| 摘 要： | 用鸡传染性法氏囊病病毒(IBDV)及其重组表达蛋白VP2,VP3和单抗做各种ELISA试验,测定单抗识别的IBDV蛋白抗原及其多肽片段,并分析单抗识别IBDV抗原表位的空间关系。结果表明,抗IBDV单抗A,B,C,D,E,G与重组表达蛋白VP2呈阳性反应,而与表达蛋白VP3呈阴性反应,单抗C与表达蛋白VP2和VP3均呈阳性反应;单抗A,B,D,E,G识别IBDV VP2蛋白上相近或相似的抗原表位,均识别VP2蛋白多肽序列PJ1,PJ2,PJ5,PJ14,属于同一类群的构像性单克隆抗体。 |
| 关 键 词： | 传染性法氏囊病病毒 衣壳蛋白 单克隆抗体 抗原表位 |
| 文章编号： | 1004-3268（2006）11-0105-05 |
| 收稿时间： | 2006-08-12 |
| 修稿时间： | 2006年8月12日 |
Analysis of Antigenic Epitopes Recognized by Anti-IBDV Monoclonal Antibody |
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| XIAO Zhi-jun,ZHANG Gai-ping,YANG Han-chun,YANG Yan-yan,LI Xue-wu,DENG Rui-guang,ZHAO Dong.Analysis of Antigenic Epitopes Recognized by Anti-IBDV Monoclonal Antibody[J].Journal of Henan Agricultural Sciences,2006(11):105-109. | |
| Authors: | XIAO Zhi-jun ZHANG Gai-ping YANG Han-chun YANG Yan-yan LI Xue-wu DENG Rui-guang ZHAO Dong |
| Institution: | 1. College of Animal Medicine,China Agriculture University. Beijing 100094,China; 2. The Biotechnology Institute of Henan Acaderny of Agricultural Sciences , Zhengzhou 450002,China |
| Abstract: | The epitopes recognized by the 5 anti-IBDV monoclonal antibodies(Mabs) of A,B,D,E and G were defined by ELISAs using Mabs,IBDV and its proteins expressed in Escherichia coli and synthetic peptides.All of the 5 Mabs reacted with recombinant VP_2 protein but not with recombinant VP_3 protein by indirect ELISA,and were shown to recognize the same or closely adjacent epitopes on IBDV VP_2 protein in ELISA additive test.The reactivity of each of the 5 Mabs with IBDV was clearly inhibited by the peptides of PJ1,PJ2,PJ5 and PJ14 respectively in antigen captured-ELISA.The results indicated that the 5 Mabs belong to one group of conformational-dependent Mabs which recognize the same or closely adjacent epitopes on VP_2 protein of IBDV,and all recognize the peptides of PJ1,PJ2,PJ5 and PJ14. |
| Keywords: | ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |