| 紫藤活性成分的提取与抑菌作用初探 |
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| 引用本文： | 姜艳华,樊晓晖,姜华.紫藤活性成分的提取与抑菌作用初探[J].河南农业科学,2009(3). |
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| 作者姓名： | 姜艳华 樊晓晖 姜华 |
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| 作者单位： | 1. 广西医科大学,微生物学与免疫学教研室,广西,南宁,530021
2. 辽宁师范大学,生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 为了测定紫藤活性成分对香瓜枯萎病菌和白菜软腐病菌的抑制作用,以不同浓度(0.1 g/mL,0.05 g/mL,0.02 g/mL,0.01 g/mL,0.005 g/mL)的紫藤叶片丙酮溶剂提取物为原料,采用牛津杯法、平板菌丝生长速率法、菌丝干重法等测定香瓜枯萎病菌和白菜软腐病菌最低抑菌浓度,以及香瓜枯萎病菌菌丝生长速率、孢子生长、菌丝干重等几项指标。结果表明,提取物浓度为0.1 g/mL时,菌丝生长最为缓慢,孢子萌发率最低,菌丝干重最小;提取物浓度为0.005 g/mL时,抑菌作用最小,为试验的最低抑菌浓度。
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| 关 键 词： | 紫藤 活性成分 抑菌作用 |
Extraction of Wisteria sinensis' Active Constituent and Its Inhibitory Effect on Fungi and Bacteria |
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| JIANG Yan-hua,FAN Xiao-hui,JIANG Hua.Extraction of Wisteria sinensis'' Active Constituent and Its Inhibitory Effect on Fungi and Bacteria[J].Journal of Henan Agricultural Sciences,2009(3). |
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| Authors: | JIANG Yan-hua FAN Xiao-hui JIANG Hua |
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| Institution: | 1.Department of Microbiology and Immunology;Guangxi Medical University;Nanning 530021;China;2.College of Life Sciences;Liaoning Normal University;Dalian 530021;China |
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| Abstract: | The active constituent was extract with acetone from the leaves of Wisteria sinensis and its effect on the withers germ of the muskmelon and the soft rot of cabbage fungus was detected.After 72 hours of extractions,different density(0.1g/mL,0.05g/mL,0.02g/mL,0.01g/mL and 0.005g/mL) was tested for Fusarium oxysporum f.sp.cubense and Erwinia carotorora and measured with the methods of oxford cup,flat growth of mycelia and dry weight of the mycelia.The results showed that when diluted 10 times(0.1g/mL),the gro... |
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| Keywords: | Wisteria sinensis Active constituent Bacteriostatic activity |
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