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| 务川黑牛微卫星座位遗传多样性分析 | |
| 引用本文： | 吴芸,蒋自立,田雄,马培江.务川黑牛微卫星座位遗传多样性分析[J].河南农业科学,2012,41(12). |
| 作者姓名： | 吴芸 蒋自立 田雄 马培江 |
| 作者单位： | 1. 遵义师范学院生物系,贵州遵义,563000 2. 务川仡佬族苗族自治县饲草饲料站,贵州务川,564300 3. 务川仡佬族苗族自治县柏村镇畜牧兽医站,贵州务川,564300 |
| 摘 要： | 以贵州地方牛优良品种务川黑牛为研究对象,选取分布于其7条染色体上的ETH10、ILSTS033、CSSM66、BM2113、ETH225、BM1818和CSRM60共7对微卫星引物进行遗传多样性分析。结果共检测到21个等位基因,每个微卫星座位等位基因数为2～5个,7个微卫星座位均未达到Hardy-Weinberg平衡。7个位点的平均等位基因数、平均有效等位基因数、平均期望杂合度、平均多态信息含量分别为3.000 0±1.154 7、2.377 3±0.585 4、0.558 4±0.101 6、0.475 9±0.125 1,说明务川黑牛具有良好的遗传多样性。为务川黑牛种质资源保存和改良提供了试验基础。 |
| 关 键 词： | 务川黑牛 微卫星 遗传多样性 遗传变异 |
The Analysis of Genetic Diversity of Microsatellite Loci in Wuchuan Black Cattle |
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| WU Yun , JIANG Zi-li , TIAN Xiong , MA Pei-jiang.The Analysis of Genetic Diversity of Microsatellite Loci in Wuchuan Black Cattle[J].Journal of Henan Agricultural Sciences,2012,41(12). | |
| Authors: | WU Yun JIANG Zi-li TIAN Xiong MA Pei-jiang |
| Abstract: | Wuchuan black cattle is a good local breed in Guizhou province.In this study,the genetic diversity of Wuchuan black cattle was analyzed by 7 selected pairs of microsatellite loci which distribute on seven different cattle chromosomes,including ETH10,ILSTS033,CSSM66,BM2113,ETH225,BM1818 and CSRM60.The results showed that 21 alleles were found,the allele numbers of each locus changes from 2 to 5 and all of these microsatellite loci didn't reached the Hardy-Weinberg equilibrium.The average number of alleles,average effective number ofalleles,average expected heterozygosity and average polymorphism information content were3.000 0±1.154 7,2.377 3±0.585 4,0.558 4±0.101 6 and 0.475 9±0.125 1,respectively.All the data represent us a good genetic diversity of Wuchuan black cattle.To provide an experimental basis for germplasm consenation and improvement of Wuchuan black cattle. |
| Keywords: | Wuchuan black cattle microsatellite genetic diversity genetic variation |
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