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谷子丝/苏氨酸蛋白激酶类抗病基因同源序列的克隆与分析
引用本文:瓮巧云,宋晋辉,张爱香.谷子丝/苏氨酸蛋白激酶类抗病基因同源序列的克隆与分析[J].河南农业科学,2012,41(6):106-108.
作者姓名:瓮巧云  宋晋辉  张爱香
作者单位:河北北方学院农林科技学院,河北张家口,075000
摘    要:根据已知丝氨酸/苏氨酸蛋白激酶(STK)类抗病基因保守结构域设计简并引物,以抗锈病谷子品种十里香和感锈病谷子品种豫谷1号为材料,利用抗病候选基因(RGA)技术对谷子STK类抗病基因同源序列进行克隆和分析,以期为谷子抗锈病相关基因的克隆及抗锈机制的研究奠定基础。结果表明,从抗病材料中获得1条STK类似序列(STK 1),长度为474bp。BLASTP分析表明,该序列含有PKc保守结构域,且与小麦抗锈病激酶Lr10、水稻丝氨酸/苏氨酸蛋白激酶受体PR5K、玉米丝氨酸/苏氨酸蛋白激酶受体1、燕麦激酶受体LRK10等同源性在71%~76%。从谷子抗锈品种十里香中获得的STK类抗病基因同源序列可能是候选的谷子抗锈病相关基因。

关 键 词:谷子  抗病候选基因  STK类抗病基因同源序列  抗锈机制

Cloning and Analysis of STK Disease Resistant Gene Analogs in Millet
WENG Qiao-yun , SONG Jin-hui , ZHANG Ai-xiang.Cloning and Analysis of STK Disease Resistant Gene Analogs in Millet[J].Journal of Henan Agricultural Sciences,2012,41(6):106-108.
Authors:WENG Qiao-yun  SONG Jin-hui  ZHANG Ai-xiang
Institution:(College of Agriculture and Forestry Science and Technology,Hebei North Universty,Zhangjiakou 075000,China)
Abstract:According to the conserved amino acid domains of STK(serine/threonine kinase) disease resistant genes,the corresponding degenerated primers were synthesized.And then the resistant gene analogs were isolated by PCR amplification from Setaria italica Beauv.cultivar Shilixiang and Yugu NO.1,which were resistant and susceptible to Uromyces setariae-italicae infection,respectively.The result showed that a piece of 474 bp STK-like sequence containing PKc conservative domain was obtained from Shilixiang.BLASTP analysis result showed that it had 71% to 76% homology with Triticum aestivum rust resistant kinase Lr10,Oryza sativa serine/threonine kinase receptor PR5K,Zea mays serine/threonine kinase receptor 1,Avena sativa kinase receptor LRK10 and so on.The STK disease resistant gene analog obtained from cultivar Shilixiang might be rust-resistant related gene in S.italica Beauv.
Keywords:Setaria italica Beauv    RGA  STK disease resistant gene analogs  rust-resistant mechanism
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