Hg~(2+)、Pb~(2+)对野生型绿豆种子萌发、幼苗生长及抗氧化酶活性的影响

为了解重金属污染对野生型绿豆和栽培型绿豆的不同影响,采用水培方法,以去离子水培养为对照(CK),研究了重金属离子Hg2+、Pb2+、(Hg2++Pb2+)污染对野生型绿豆E和栽培型绿豆P种子萌发、幼苗生长及抗氧化酶活性的影响。结果表明:Hg2+、Pb2+和(Hg2++Pb2+)污染均能抑制绿豆种子的萌发和幼苗的生长,伤害程度为(Hg2++Pb2+)>Hg2+>Pb2+,其中(Hg2++Pb2+)质量浓度为(2.0+100)mg/L时,品种P的发芽率、幼苗苗高和根长分别比其CK降低25.8%、48.1%和79.7%,品种E分别比其CK降低22.4%、44.7%、70.0%;一定质量浓度的Hg2+、Pb2+、(Hg2++Pb2+)污染下,绿豆叶片内的抗氧化酶活性提高,其中(Hg2++Pb2+)质量浓度为(2.0+100)mg/L时,品种P叶片内SOD、POD、CAT的活性比其CK分别增加了113.7%、304%、254%,品种E叶片内SOD、POD、CAT的活性比其CK分别增加了131.0%、333%和278%。可见,Hg2+、Pb2+、(Hg2++Pb2+)胁迫下野生绿豆E品种的萌发能力、幼苗生长及其抗氧化能力均高于栽培绿豆P品种。

Effects of Hg2+ and Pb2+ on Seed Germination,Growth and Antioxidant Enzymes Activities of Wild Type Mung Bean

In order to investigate the different effects of heavy metal pollution on wild and cultivated-type mung bean,effects of pollution of single and combined heavy metal ions of Hg2+,Pb2+,Hg2+ and Pb2+ on the seed germination,growth and antioxidant enzymes activities of mung bean seedling were studied by hydroponics culture in pots,with the deionized water culture as control(CK).The results showed that the seed germination and seedling growth were inhibited under the pollution of single and combined ions of Hg2+ and Pb2+,followed by(Hg2+ +Pb2+)>Hg2+ >Pb2+.The germination percentage,seedting length and root length of type P mung bean reduced by 25.8%,48.1% and 79.7% compared with the control under(2.0+100) mg/L of(Hg2+ +Pb2+) stress,and these indicators of wild type mung bean reduced by 22.4%,44.7% and 70.0% respectively.In addition,the antioxidant enzymes activities of leaf were increased observably after the heavy metal treatment.The activities of SOD,POD and CAT of type P mung bean increased by 113.7%,304% and 254% under(2.0+100) mg/L of(Hg2+ +Pb2+) stress,and the activities of SOD,POD and CAT of wild type mung bean increased by 131%,333% and 278% compared respectively with the control.It was concluded that the seed germination,growth and antioxidant enzymes activities of wild type mung bean were more significant than those of the type P mung bean under Hg2+,Pb2+ and(Hg2+ +Pb2+) stress.