| 培养基成分对黄花蒿丛生芽合成青蒿素的影响 |
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| 引用本文： | 刘春朝,尚富德.培养基成分对黄花蒿丛生芽合成青蒿素的影响[J].河南农业科学,2011,40(1):131-133. |
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| 作者姓名： | 刘春朝 尚富德 |
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| 作者单位： | 1. 河南中医学院,药学院,河南,郑州,450003
2. 河南大学,生命科学学院,农业生物技术研究所,河南,开封,475001 |
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| 摘 要： | 以MS为基本培养基,研究了MS培养基中大量元素浓度对黄花蒿丛生芽合成青蒿素的影响,确定了最适的培养条件.结果表明,青蒿素丛生芽合成青蒿素的最适条件为:蔗糖质量浓度为30g/L,铵盐和硝酸盐比值为1:3,氮源浓度为45mmol/L,无机磷酸盐质量浓度为200mg/L.
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| 关 键 词： | 黄花蒿 丛生芽 青蒿素 |
The Effects of Culture Medium Component on Artemisinin Synthesizing in Cluster Shoots of Artemisia annua L. |
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| LIU Chun-chao,SHANG Fu-de.The Effects of Culture Medium Component on Artemisinin Synthesizing in Cluster Shoots of Artemisia annua L.[J].Journal of Henan Agricultural Sciences,2011,40(1):131-133. |
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| Authors: | LIU Chun-chao SHANG Fu-de |
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| Institution: | 1.Pharmacy College,Henan University of Traditional Chinese Medicine,Zhengzhou 450003,China;2.College of Life Science,Institute of Agricultural Biotechnology,Henan University,Kaifeng 475001,China) |
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| Abstract: | The effects of culture medium component on artemisinin synthesizing in cluster shoots of Artemisia annua L.were investigated.The treatments were designed based on MS medium and the suitable cultivating conditions were confirmed.The results was follows: the suitable condition for artemisinin synthesizing in cluster shoots of Artemisia annua L.was 30g/L sucrose,NH+4-N∶NO-3-N= 1∶3,45 mmol/L nitrogen source,and 200mg/L inorganic phosphate. |
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| Keywords: | Artemisia annua L Cluster shoots Artemisinin |
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