离子束介导遗传转化小麦突变体的遗传分析

以离子束介导后代的9个小麦突变体和4个受体作供试材料,对其进行了RAPD和SSR分子标记,在此基础上获得了矮秆突变株系1042的2个特异片断,并进行了测序和同源序列分析。13个材料间分子标记遗传差异比较结果表明,离子束介导遗传转化后的突变明显小于小麦品种间的遗传差异。矮秆突变株系1042的SSR标记特异片断序列BLAST分析结果表明,从1042中扩增到的特异片断与小麦高秆基因Rht-D1 a中的部分序列高度一致,1042的株高明显降低可能是由于该株系遗传突变,产生了2个矮秆基因所致;该特异片断与小麦BAC克隆中LTR-retrotransposon Angela-3s转座子的部分片断同源性最高,说明离子束介导遗传转化诱发变异可能与小麦基因组中反转录转座子的激活有关。

Genetic Analysis of Wheat Mutants by Ion Beam-mediated Transformation

Nine wheat mutants from genetic transformation mediated by ion beam and their 4 recipient varieties were taken as test materials to conduct RAPD and SSR marker analysis,and then 2 special fragments from the dwarf mutant 1042 were sequenced,homology analysis of which was done.The results showed that the variance of mutants from genetic transformation byt ion beam was smaller than the genetic variance between different recipient varieties.After BLAST analysis,the special fragments of SSR marker from the dwarf mutant 1042 were highly identical to partial sequence of high-straw gene Rht-D1a,suggesting that the dwarfing might be caused by the two mutation dwarf genes;while the special fragments of RAPD marker had high homology with partial sequence of LTR-retrotransposon Angela-3s in wheat BAC clones,which suggested that the mechanism of mutagenesis by ion beam is partially attributed to the re-activation of the retrotransposons.