不同叶色石榴杂种单株叶片色素含量的比较

为了研究石榴杂种后代不同叶片颜色的成因,采用分光光度法对8个石榴杂种单株和2个亲本(母本豫大籽、父本突尼斯软籽)对照单株生长期叶片中叶绿素、类胡萝卜素和花青素含量进行了研究。结果表明,在生长初期,叶片表现红色的4个单株花青素含量均高于对照,其中5号单株花青素含量最高,达到4.12mg/g;叶片表现黄绿色的4个单株叶绿素和类胡萝卜素含量的变化趋势与对照基本一致,其中4号单株叶绿素含量和叶绿素/类胡萝卜素的值最高,而3号单株叶绿素a/b最低。在生长旺盛期,8个石榴单株叶绿素、类胡萝卜素和花青素含量的变化趋势一致,与对照无明显差异。经方差分析,不同石榴单株在不同的生长期其叶绿素和类胡萝卜素含量均存在显著差异,不同石榴单株间花青素含量存在显著差异,而不同石榴单株在不同的生长期其叶绿素/类胡萝卜素的值无显著差异,可以初步得出石榴杂种后代间叶片颜色不同是由于生长期花青素、叶绿素含量和比值不同造成的。

Determination and Comparison of Leaf Pigment Content of Punica granatum L.Hybrid with Different Leaf Color

Spectrophotometry was used to study the contents of three pigments,chlorophyll,carotenoid,and anthocyanin,in the leaves of eight pomegranate trees and their parents.The results showed that at the beginning of growing period,the anthocyanin content of the four pomegranate trees with red leaves was higher than the control tree.The anthocyanin content of No.5 individual plant was the highest(4.12 mg/g).The dynamic trends of chlorophyll and carotenoids of the four pomegranate trees with green yellow leaves were consistent with the contrast tree.The chlorophyll content and the chlorophyll/carotenoids content of No.4 were the highest,the chlorophyll a/b content of No.3 was the lowest.During vigorous growing period,the dynamic trends of pigments including chlorophyll,carotenoid and anthocyanin of eight individual plants were consistent;there was no significant difference comparing with the control.Analysis of variance indicated that there was a significant difference in the contents of chlorophyll and carotenoid between different trees in different growth periods and the content of anthocyanin between different trees,while the value of chlorophyll/carotenoids between different trees was not obvious.