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| ZmNAP基因对玉米抗衰老及产量性状的调控效应 | |
| 引用本文： | 张永辉,曹言勇,邵强,王利锋,王浩,李晶晶,李会勇.ZmNAP基因对玉米抗衰老及产量性状的调控效应[J].河南农业科学,2012,41(10):19-24. |
| 作者姓名： | 张永辉 曹言勇 邵强 王利锋 王浩 李晶晶 李会勇 |
| 作者单位： | 1. 河南师范大学生命科学学院,河南新乡453007;河南省农业科学院粮食作物研究所,河南郑州450002 2. 河南省农业科学院粮食作物研究所,河南郑州,450002 3. 河南师范大学生命科学学院,河南新乡,453007 |
| 基金项目： | 国家转基因生物新品种培育重大专项 |
| 摘 要： | 为了探索ZmNAP基因在调控玉米抗衰老及产量性状等方面的作用,根据已知的ZmNAP基因cDNA序列构建玉米Ubi启动子控制下的ZmNAP的RNA干扰载体pTCK303ihpZmNAP,并通过农杆菌介导的方法对ZmNAP基因进行遗传转化。结果表明:ZmNAP转基因玉米植株在叶片滞绿、籽粒千粒重等方面较野生型植株(WT)有明显优势,转基因植株比野生型植株千粒重提高15%～30%。ZmNAP基因在玉米育种中具有重要的应用价值。 |
| 关 键 词： | 玉米 ZmNAP基因 RNAi 抗衰老 滞绿性 千粒重 |
Regulating Effect of ZmNAP Gene on Anti-senescence and Yield Traits of Maize |
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| ZHANG Yong-hui , CAO Yan-yong , SHAO Qiang , WANG Li-feng , WANG Hao , LI Jing-jing , LI Hui-yong.Regulating Effect of ZmNAP Gene on Anti-senescence and Yield Traits of Maize[J].Journal of Henan Agricultural Sciences,2012,41(10):19-24. | |
| Authors: | ZHANG Yong-hui CAO Yan-yong SHAO Qiang WANG Li-feng WANG Hao LI Jing-jing LI Hui-yong |
| Institution: | 1.College of Life Sciences,Henan Normal University,Xinxiang 453007,China; 2.Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
| Abstract: | In order to investigate the potential role of ZmNAP in regulating the anti-senescence and yield traits of maize,the maize ZmNAP RNA interference vector pTCK303ihpZmNAP driven by Ubi promoter was created based on the ZmNAP cDNA sequence deposited in MaizeGDB,and was transformed into maize by Agrobacterium tumefaciens.The results showed that transgenic maize plants had more obvious advantages than control plants in delaying senescence and grain weight.The 1 000-grain weight of transgenic maize plants was 15%-30% higher than that of wild type maize plants.ZmNAP gene has potential value in maize breeding. |
| Keywords: | maize ZmNAP gene RNAi delaying senescence stay green 1 000-grain weight |
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