| 口蹄疫抗体免疫层析试纸的临床应用及性能评价 |
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| 引用本文： | 杨苏珍,杨继飞,李清州,卢清侠,万博,鲍登克,刘运超,柴书军,张改平.口蹄疫抗体免疫层析试纸的临床应用及性能评价[J].河南农业科学,2012,41(10):148-152. |
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| 作者姓名： | 杨苏珍 杨继飞 李清州 卢清侠 万博 鲍登克 刘运超 柴书军 张改平 |
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| 作者单位： | 1. 河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州,450002
2. 河南省农业科学院农业经济与信息研究中心,河南郑州,450002 |
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| 基金项目： | 农业部公益性行业专项，河南省农业科学院科研发展专项 |
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| 摘 要： | 为评价前期研制的猪口蹄疫O型抗体检测试纸的临床应用效果,检测了162头份口蹄疫病毒(FMDV)多肽疫苗免疫猪血清和87头份灭活疫苗免疫猪血清,并与UBI FMDV抗体检测ELISA和美国BioXL FMDV抗体检测ELISA做平行对比试验。结果显示,试纸与2种ELISA方法的符合率分别为95.06%和96.55%。说明该试纸可以用于猪FMDV O型抗体的临床检测,为评价口蹄疫疫苗免疫效果提供了快速、简便的方法。
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| 关 键 词： | 口蹄疫病毒 免疫层析试纸 ELISA |
Clinical Application and Evaluation of the Immunochromatographic Strip for Detecting Antibodies against Swine Foot-and-mouth Disease Virus |
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| YANG Su-zhen
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YANG Ji-fei
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LI Qing-zhou
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LU Qing-xia
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WAN Bo
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BAO Deng-ke
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LIU Yun-chao
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CHAI Shu-jun
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ZHANG Gai-ping.Clinical Application and Evaluation of the Immunochromatographic Strip for Detecting Antibodies against Swine Foot-and-mouth Disease Virus[J].Journal of Henan Agricultural Sciences,2012,41(10):148-152. |
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| Authors: | YANG Su-zhen YANG Ji-fei LI Qing-zhou LU Qing-xia WAN Bo BAO Deng-ke LIU Yun-chao CHAI Shu-jun ZHANG Gai-ping |
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| Institution: | 1*(1.Henan Provincial Key Laboratory of Animal Immunology/Key laboratory of Animal Immunology of the Ministry of Agriculture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;2.Agricultural Economy and Information Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
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| Abstract: | To evaluate the clinical application effects of the immunochromatographic strips for detecting antibodies against swine foot-and-mouth disease virus(FMDV),a total of 162 sera samples from the FMDV-peptide vaccine immunized pigs and 87 samples from the FMDV inactivated vaccine immunized pigs were detected by the immunochromatographic strips and FMDV antibody detecting ELISA kits provided by UBI or BioXL simultaneously.The results showed that the agreement rate of the strip with the two ELISA kits was 95.06% and 96.55%,respectively.This suggests that the strip can be used for detecting FMDV clinical serum samples and displays rapid and simple features. |
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| Keywords: | FMDV immunochromatographic strip ELISA |
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