HPLC法鉴别铁棍山药与太谷山药

采用高效液相色谱(HPLC)法研究铁棍山药与太谷山药特征图谱,通过索氏提取法、蒸馏法和超声提取法提取并比较2种山药间成分组成及其含量差异,为铁棍山药的品种鉴定提供依据。使用Agilent ODS色谱柱(5μm,4.6mm×250mm),以甲醇-水(95∶5)为流动相,流速1.0mL/min,柱温30℃,检测波长215nm,对2种山药的成分进行HPLC分析。结果表明,超声提取法效果较好。2种山药图谱峰的个数和出峰时间有一定区别,峰面积有明显差别,可以做为指纹图谱来甄别2种山药,所建立的方法可用于铁棍山药的品种鉴定。铁棍山药和太谷山药中均没有检测到皂苷元。

Identification of Dioscorea oppostia Thunb.cv.Tiegun and D.oppostia Thunb.cv.Taigu by HPLC

To evaluate the quality of Dioscorea oppostia Thunb.cv.Tiegun,HPLC method was established for the species identification by comparing the composition and content between D.oppostia Thunb.cv.Tiegun and D.oppostia Thunb.cv.Taigu,which was obtained respectively by Soxhlet extraction,distillation extraction,and ultrasonic extraction technology.The analysis was performed on a Agilent ODS-C18 column(5 μm,4.6 mm×250 mm),using methanol-water(95∶5) as the mobile phase,with gradient elution,ata flow rate of 1.0 mL/min,ata column temperature of 30 ℃,and the detection wavelength at 215 nm.The results showed that ultrasonic extraction technology was better than the other two extraction technology.There were some differences between D.oppostia Thunb.cv.Tiegun and D.oppostia Thunb.cv.Taigu in the number and time of peaks,and the area of peak in D.oppostia Thunb.cv.Taigu was more than that in D.oppostia Thunb.cv.Tiegun.This meathod could be used to identify D.oppostia Thunb.cv.Tiegun.Sapogenin was not found in the two kinds of yams.