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126种中草药提取物对2种植物病原真菌的抑制作用
引用本文:王树桐,张凤巧,高瑞平,甄文超,鲍时翔,蒋继志,曹克强.126种中草药提取物对2种植物病原真菌的抑制作用[J].河南农业科学,2006(10):62-65.
作者姓名:王树桐  张凤巧  高瑞平  甄文超  鲍时翔  蒋继志  曹克强
作者单位:1. 河北农业大学植物保护学院,河北省作物病虫害生物防治工程技术中心,河北,保定,071001;中国热带农业科学院生物技术研究所,热带作物生物技术国家重点实验室,海南,海口571101
2. 河北农业大学图书馆,河北,保定,071001
3. 河北农业大学植物保护学院,河北省作物病虫害生物防治工程技术中心,河北,保定,071001
4. 中国热带农业科学院生物技术研究所,热带作物生物技术国家重点实验室,海南,海口571101
基金项目:科技部粮食丰产科技工程项目;河北农业大学校科研和教改项目
摘    要:以禾谷镰刀菌(Fusarium graminearum)和立枯丝核菌(Rhizoctonia solani)为供试菌种,中草药提取物样品供试质量浓度为0.01g/ml,用生长速率法对126种中草药的乙醇提取物进行了室内抑菌活性测试。结果表明,在供试的126种中草药提取物中,16种提取物对禾谷镰刀菌菌丝生长具有显著的抑制作用,且抑菌作用大于50%,甘草提取物的抑菌活性达86.99%。将这16种抑菌活性较好的提取物稀释200倍进一步测试,有7种提取物抑菌作用大于50%。供试的126种中草药提取物中有23种提取物对立枯丝核菌菌丝生长具有显著的抑制作用,且抑菌作用大于50%,鸡冠花提取物的抑菌率达到100%。二次筛选后仍有8种提取物的抑菌活性大于60%,其中鸡冠花提取物的抑菌率仍为100%。

关 键 词:中草药提取物  禾谷镰刀菌  立枯丝核菌  抑菌活性
文章编号:1004-3268(2006)10-0062-04
收稿时间:2006-04-17
修稿时间:2006年4月17日

Inhibition of 126 Kinds of Chinese Herb Extracts Gainst Two Plant Pathogenic Fungi
WANG Shu-tong,ZHANG Feng-qiao,GAO Rui-ping,ZHEN Wen-chao,BAO Shi-xiang,JIANG Ji-zhi,CAO Ke-qiang.Inhibition of 126 Kinds of Chinese Herb Extracts Gainst Two Plant Pathogenic Fungi[J].Journal of Henan Agricultural Sciences,2006(10):62-65.
Authors:WANG Shu-tong  ZHANG Feng-qiao  GAO Rui-ping  ZHEN Wen-chao  BAO Shi-xiang  JIANG Ji-zhi  CAO Ke-qiang
Institution:1. Bio-control Center of Plant Disease and Pests of Hebei Province,College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China; 2. State Key Laboratory of Tropical Crop Biotechnology,Institute Bioscience and Biotechnology Chinese Academy for Tropical Agricultural Sciences, Haikou 571101 ,China; 3. Library of Agricultural University of Hebei, Baoding 071001,China
Abstract:The extracts from hundred and twenty six species of Chinese herbs(CHEs) were tested for the antifungal activities against the mycelium growth of Fusarium graminearum and Rhizoctonia solani.The concentration 0.01 g/ml was used in the primary screening.The results showed that 16 species of CHEs expressed significant inhibitory effects against the mycelium growth of Fusarium graminearum,and the inhibitory effect was over 50%.Inhibitory rate of Glycyrrhiza yralensis extract reached up to 87%.Inhibitory rates of 7 species of CHEs were more than 50% after diluted 200 times at the secondary test.At the primary screening test,23 species of CHEs had significant inhibitory effects against the mycelium growth of Rhizoctonia solani with the inhibitory rates over 50%.Inhibitory effect of Celosia cristata L.extract reached up to 100%.The inhibitory effects of 8 species in these 23 species of CHEs were still higher than 60% and the effect of Celosia cristata L.extract did not reduce at the secondary screening test.
Keywords:Chinese herb extract  Fusarium graminearum  Rhizoctonia solani  Antifungal activity
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