河南省部分地区鸡志贺氏菌病病原分离鉴定及药敏试验

为了对河南省部分地区鸡志贺氏菌病的病原进行分离鉴定和药物敏感性调查,对河南省6个地区的鸡腹泻病病料进行了细菌学检查,经细菌分离培养、形态染色镜检、生化试验和动物试验,共分离鉴定出12株鸡源志贺氏菌。血清学试验结果显示,分离菌株存在种和型的差异,其中8株为福氏志贺氏菌,3株为鲍氏多价志贺氏菌,1株为痢疾Ⅱ型志贺氏菌。动物试验结果表明,分离的12株鸡志贺氏菌均具有致病性,且毒力不同,其中,从三门峡分离的菌株有很强的毒力,发病率和死亡率均为100%。药敏试验结果显示,大多数鸡志贺氏菌对氨苄/舒巴坦和阿米卡星较为敏感,对其他药物具有严重的耐药性,且不同地区分离的鸡志贺氏菌对药物的敏感性不同。

Isolation and Indentification of Shigellae from Chicken in Some Regions of Henan Province and Their Susceptibility Tests to Drugs

In order to indentify and test drugs sensitivity to the Shigellae from chicken in some region of Henan province,the bacteria for the diseased diarrhea from six regions in Henan province were examined in this study.Isolating,observations of bacteria appearance,cultivating characteristics and biochemical characteristics in addition to animal testing finally showed that twelve bacteria belong to Shigellae.Serological typing test were performed and showed that these strains have differences in species and types.The results revealed that eight strains were identified for S.flexneri,three strains were identified for S.boydii,and the other one strain was identified for Ⅱ type S.dysenteriae infection.Animal experiments suggested that the tweleve Shigellae are pathogenic but the virulence are different.The strain from Sanmenxia is virulent and the morbidity and mortality are both 100%.The sensitivity tests to drugs show that most Shigellae isolated from chicken are sensitive to ampicillin/sulbactam and amikacin,but showed serious resistant to other drugs.The susceptibilities are different among the Shigellae isolates from different regions.The study provides a scientific basis for Shigella bacteria on chicken diagnosis and treatment of disease.