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| 白菜型油菜BrDAD1基因的克隆及其植物反义载体的构建 | |
| 引用本文： | 吴晓梅,朴学成,陈明训,王中.白菜型油菜BrDAD1基因的克隆及其植物反义载体的构建[J].河南农业科学,2010(5). |
| 作者姓名： | 吴晓梅 朴学成 陈明训 王中 |
| 作者单位： | 1. 丽水学院,生物系,浙江,丽水,323000 2. 浙江大学,农业与生物技术学院,作物科学研究所,浙江,杭州,310029;金日成综合大学,生命科学学院,遗传系,朝鲜,平壤 3. 浙江大学,农业与生物技术学院,作物科学研究所,浙江,杭州,310029 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 应用聚合酶链式反应技术(PCR)扩增了白菜型油菜BrDAD1基因及自身的启动子基因Pro-DAD1,并将其分别克隆到pMD18-Tvector载体上,对重组子进行PCR检测和限制性内切酶分析,并测定了该基因及启动子全序列。结果表明,该基因及启动子全长分别为1 270 bp与1 335 bp。将白菜型油菜BrDAD1基因反向克隆到植物表达载体pCambia 1300的自身启动子基因Pro-DAD1启动子下游,构建了该基因的植物反义表达载体pCamDAD1。 |
| 关 键 词： | 油菜 DAD1 启动子 反义表达载体 |
Cloning and Construction of Plant Antisense Expression Vector Containing BrDAD1 Gene in Brassica rapa |
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| WU Xiao-mei,PaK Haksong,CHEN Ming-xun,WANG Zhong.Cloning and Construction of Plant Antisense Expression Vector Containing BrDAD1 Gene in Brassica rapa[J].Journal of Henan Agricultural Sciences,2010(5). | |
| Authors: | WU Xiao-mei PaK Haksong CHEN Ming-xun WANG Zhong |
| Abstract: | BrDAD1 gene and its promoter Pro-DAD1 in Brassica rapa were obtained by PCR technique and cloned into pMD18-Tvector respectively.The recombinant clone were detected by PCR technique and analyzed by the restriction enzyme.Two full lengths of BrDAD1 and Pro-DAN1 were sequenced.The results showed that the lengths of the clone sequences were 1270 and 1335bp respectively.BrDAD1 gene in Brassica rapa was reversely cloned into its promoterPro-DAN1 downstream of plant expression vector pCambia 1300 in orientation,and then antisense expression vector pCamDAD1 has been constructed. |
| Keywords: | DAD1 |
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