| 欧李茎尖分生组织培养与快速繁殖技术研究 |
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| 引用本文： | 王正德,庞发虎.欧李茎尖分生组织培养与快速繁殖技术研究[J].河南农业科学,2006(9):97-99. |
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| 作者姓名： | 王正德 庞发虎 |
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| 作者单位： | 南阳师范学院生物系,河南,南阳,473000 |
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| 基金项目： | 河南省南阳市科技攻关计划;南阳师范学院校科研和教改项目 |
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| 摘 要： | 以欧李茎尖分生组织为外植体,研究了在培养基中添加不同的激素成分及不同浓度对不定芽诱导、继代增殖和生根培养的影响。结果表明:不定芽诱导的最佳培养基是MS 6-BA0.4mg/L IAA0.5mg/L;增殖培养的最适培养基为MS 6-BA0.3mg/L IAA0.4mg/L;最佳生根培养基是2/3MS IAA0.5mg/L。应用症状诊断和指示植物鉴定法进行病毒检测,平均脱毒率为73%。
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| 关 键 词： | 欧李 组织培养 脱毒 快速繁殖 |
| 文章编号： | 1004-3268（2006）09-0097-03 |
| 收稿时间： | 2006-04-17 |
| 修稿时间： | 2006年4月17日 |
Technology System for Stem-apex Meristem Culture and Rapid Propagation of Prunus humilis |
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| WANG Zheng-de,PANG Fa-hu.Technology System for Stem-apex Meristem Culture and Rapid Propagation of Prunus humilis[J].Journal of Henan Agricultural Sciences,2006(9):97-99. |
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| Authors: | WANG Zheng-de PANG Fa-hu |
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| Institution: | Biological Department of Nanyang Normal College, Nanyang 473000, China |
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| Abstract: | The influences of the factors such as composition and concentration of hormones in culture medium upon adventitious bud induction, shoot proliferation and rooting were studied with the stem-apex meristem of Prunus humilis as explant. The results showed that the optimum medium for adventitious bud regeneration was MS 6-BA 0.4 mg/L IAA 0.5 mg/L; the best culture medium for shoot proliferation was MS 6-BA 0.3 mg/L IAA 0.4 mg/L; the proper culture medium of rooting was 2/3MS IAA 0.5 mg/L. And the average virus-free rate was 73% based on the semiological diagnosis and plant indicator identification. |
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| Keywords: | Prunus humilis Tissue culture Virus-free Rapid propagation |
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