| 烟草花粉管通道导入外源DNA研究初探 |
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| 引用本文： | 邓明军,崔红.烟草花粉管通道导入外源DNA研究初探[J].河南农业科学,2005(1):22-24. |
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| 作者姓名： | 邓明军 崔红 |
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| 作者单位： | 国家烟草栽培生理生化研究基地,河南,郑州,450002;国家烟草栽培生理生化研究基地,河南,郑州,450002 |
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| 基金项目： | 国家烟草专卖局科技项目资助 (110 2 0 0 2 0 10 0 2 ) |
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| 摘 要： | 以不同浓度的 pIG12 1Hm质粒DNA为供体 ,在烟草授粉后不同时期 ,采用不同的方法经花粉管通道导入 ,收获的种子经卡那霉素 (Km )抗性筛选。结果表明 ,在授粉后 10h ,DNA浓度为5 0 0 μg/ml,采用注射的方法可得到最大转化率 (2 3.5 % )。
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| 关 键 词： | 烟草 花粉管通道 质粒 卡那霉素抗性 |
| 文章编号： | 1004-3268(2005)01-0022-03 |
| 修稿时间： | 2004年6月11日 |
Primary Study on Exogenous DNA Introduction into Tobacco Via the Pollen-tube Pathway |
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| DENG Ming-Jun,CUI Hong.Primary Study on Exogenous DNA Introduction into Tobacco Via the Pollen-tube Pathway[J].Journal of Henan Agricultural Sciences,2005(1):22-24. |
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| Authors: | DENG Ming-Jun CUI Hong |
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| Institution: | DENG Ming Jun,CUI Hong * |
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| Abstract: | Plasmid pIG121Hm containing report gene gus A and plant selected gene npt II was introduced into tobacco`Basma'via the pollen tube pathway at different time with different concentration by different ways. Seeds were harvested and screened in 500 μg/ml kanamycine solution.Highest ratio(23.5%) of gene transformation was discovered when 500 μg/ml plasmid pIG121Hm DNA was introduced into ovary for 10 hours after pollination by injection. |
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| Keywords: | Tobacco Pollen-tube pathway Plasmid Kanamycine resistance |
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