| 恩诺沙星单克隆抗体杂交瘤细胞株的建立及其免疫学特性 |
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| 引用本文： | 赵银丽,滕蔓,邓瑞广,柴书军,刘庆堂,张改平.恩诺沙星单克隆抗体杂交瘤细胞株的建立及其免疫学特性[J].河南农业科学,2008(10). |
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| 作者姓名： | 赵银丽 滕蔓 邓瑞广 柴书军 刘庆堂 张改平 |
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| 作者单位： | 1. 河南省农业科学院;河南省动物免疫学重点实验室,河南,郑州 450002;河南工业大学生物工程学院,河南,郑州,450001
2. 河南省农业科学院;河南省动物免疫学重点实验室,河南,郑州450002 |
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| 摘 要： | 将恩诺沙星(ENR)偶联于载体蛋白BSA,形成完全抗原(BSA-ENR)并免疫BALB/c小鼠,应用细胞融合技术制备分泌抗恩诺沙星的杂交瘤细胞株,用体内诱生腹水法生产ENR单克隆抗体。结果筛选出4G1-B3,4G1-G1和4G1-B9 3株敏感特异的杂交瘤。3株单抗对ENR的半数抑制浓度(IC50)分别为1.04 ng/mL,2.44 ng/mL,4.24 ng/mL。4G1-B3与环丙沙星有0.02%的交叉反应,与其他竞争物的交叉反应率均小于0.01%;4G1-G1和4G1-B9与其他竞争物的交叉反应率均小于0.01%。
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| 关 键 词： | 恩诺沙星 杂交瘤 单克隆抗体 免疫学特性 |
Generation and Characterization of Monoclonal Antibodies against Enrofloxacin |
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| ZHAO Yin-li,TENG Man,DENG Rui-guang,CHAI Shu-jun,LIU Qing-tang,ZHANG Gai-ping.Generation and Characterization of Monoclonal Antibodies against Enrofloxacin[J].Journal of Henan Agricultural Sciences,2008(10). |
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| Authors: | ZHAO Yin-li TENG Man DENG Rui-guang CHAI Shu-jun LIU Qing-tang ZHANG Gai-ping |
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| Abstract: | In this study,monoclonal antibodies(mAbs) against enrofloxacin(ENR),which was conjugated with bovine sera albumin(BSA),were produced by using conventional cell fusion technology.Three high affinity hybridoma cell lines,4G1-B3,4G1-G1 and 4G1-B9,were finally screened specific to ENR by competitive inhibition enzyme-linked immunosorbent assay(ELISA).The 50% inhibiting concentrations(IC50) of these three mAbs were 1.04ng/mL,2.44ng/mL and 4.24ng/mL to ENR,respectively.The cross-reactivity of 4G1-B3 mAb to ciprofloxacin is 0.02 % and less than 0.01% to other competitors,while that of 4G1-G1 and 4G1-B9 mAbs to all tested competitors are less than 0.01%. |
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| Keywords: | Enrofloxacin Hybridoma cell line Monoclonal antibody Immunologic characteristic |
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