| 应用AFLP标记筛选甘蓝型油菜R4和22B的多态性 |
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| 引用本文： | 高玉千,张书芬,朱家成,张世敏,戚元成.应用AFLP标记筛选甘蓝型油菜R4和22B的多态性[J].河南农业科学,2005(8):32-35. |
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| 作者姓名： | 高玉千 张书芬 朱家成 张世敏 戚元成 |
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| 作者单位： | 1. 河南农业大学生命科学学院,河南,郑州450002
2. 河南省农业科学院棉花油料作物研究所,河南,郑州450002 |
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| 基金项目： | 国家“十五”科技攻关项目(2001BA511B07)，河南省杰出青年基金项目(0212000700) |
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| 摘 要： | 应用AFLP分子标记,对甘蓝型油菜恢复系R4和保持系22B进行引物多态性筛选。在414对AFLP引物中,初步筛选到有多态性的引物组合147对;其中重复性好、多态性比率高的引物组合120对;每对引物可扩增出50条左右的条带,其中,多态性条带数为12.4条。AFLP是构建图谱中效率较高的分子标记。
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| 关 键 词： | 甘蓝型油菜 AFLP 多态性 筛选 |
| 文章编号： | 1004-3268(2005)08-0032-04 |
| 收稿时间： | 2005-04-16 |
| 修稿时间： | 2005年4月16日 |
Selection of Polymorphism of Brassica napus L R4 and 22B by AFLP Analysis |
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| GAO Yu-qian,ZHANG Shu-fen,ZHU Jia-cheng,ZHANG Shi-min,QI Yuan-cheng.Selection of Polymorphism of Brassica napus L R4 and 22B by AFLP Analysis[J].Journal of Henan Agricultural Sciences,2005(8):32-35. |
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| Authors: | GAO Yu-qian ZHANG Shu-fen ZHU Jia-cheng ZHANG Shi-min QI Yuan-cheng |
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| Abstract: | AFLP analysis were used in R4 and 22B, two parental lines in Brassica napus L. From the primary survey of 414 AFLP primer combinations, 147 AFLP primer combinations with polymorphism were selection, in which 120 AFLP primer combinations showed a high polymorphism ratio and a good repeatability. The bands amplified by each primer combination were 50, in which the polymorphism bands was 12.4. Based on the results, it can be concluded that high quality map will be obtained by AFLP molecular markers. |
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| Keywords: | Brassica napus L AFLP Polymorphism Selection |
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