纤维素酶产生菌的筛选及其相互作用研究

从森林落叶土、腐烂的秸秆和农家堆肥等含有木质纤维素降解菌的样品中分离到能较好降解纤维素的菌株4株,初步判定为3株细菌,1株放线菌。各菌株产酶(CMCase)较适宜的温度均是37℃,培养基初始pH值7.0,接种量(V/V)2%,接种培养12 h的种子液酶活较高。对各菌株单独与混合培养研究表明:菌株组合D6/D7、D1/D7、D6/D7/B7和D1/D6/D7/B7在72 h的酶活均显著高于其单一菌株,其中最优组合D6/D7在72 h的酶活可达67.12 U/mL,相当于其菌株单独培养酶活的2倍。

Screening of CMCase Producing Microorganisms and Their Interaction

Four strains of microbes with higher cellulase activity were selected from the samples of forest defoliation soil,rotten stalk and farmhouse compost at primary examination.Three were known as bacteria,one was known as Actinomyces.Their optimum enzyme-producing conditions were 37℃,medium primary pH at 7.0,and 2% of inoculation,inoculum age at 12h.These strains of microbes were also cultured solely and mixedly.The results showed that CMC enzyme activity at 72h of D6/D7,D1/D7,D6/D7/B7 and D1/D6/D7/B7 were obviously higher than that of the single strain,in which D6/D7 was the best,its enzyme activity reached 67.12U/mL,equal to double of each single strain.This study provided theoretical basis for combination of superior strains and their co-operation in gene engineering.