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棉铃虫对Bt毒素抗性遗传方式的研究
引用本文:李国平,吴孔明,封洪强,邱峰,郭予元.棉铃虫对Bt毒素抗性遗传方式的研究[J].河南农业科学,2008(10).
作者姓名:李国平  吴孔明  封洪强  邱峰  郭予元
作者单位:1. 河南省农业科学院植物保护研究所,河南,郑州450002
2. 中国农业科学院,植物保护研究所,北京,100094
基金项目:河南省科技攻关项目,国家重点实验室基金,河南农科院博士启动基金
摘    要:在室内条件下,采用对Bt毒素产生253倍的抗性棉铃虫种群,研究其对Cry1Ac的抗性遗传方式。结果表明,互交后代(抗性雄蛾×敏感雌蛾和抗性雌蛾×敏感雄蛾)的效应显性值DML分别为0.14和0.11,二者差异不显著,表明抗性为常染色体,不完全隐性遗传;对正反交F1代的回交后代、正反交F1代的自交后代生长发育期望值与实际值进行χ2分析,表明抗性由单基因控制。

关 键 词:Bt毒素  转基因棉花  棉铃虫  抗性遗传

Inheritance of Resistance to Bt Toxin in Helicoverpa armigera(Lepidoptera: Noctuidae)
LI Guo-ping,WU Kong-ming,FENG Hong-qiang,QIU Feng,GUO Yu-yuan.Inheritance of Resistance to Bt Toxin in Helicoverpa armigera(Lepidoptera: Noctuidae)[J].Journal of Henan Agricultural Sciences,2008(10).
Authors:LI Guo-ping  WU Kong-ming  FENG Hong-qiang  QIU Feng  GUO Yu-yuan
Abstract:Inheritance of resistance to the Bacillus thuringiensis(Bt) crystal protein was studied in Cotton bollworm(CBW) Helicoverpa armigera(Hubner),a population with 253-fold resis-tance to Cry1Ac than a control susceptible strain.The progeny of reciprocal F1 crosses(resistant male×susceptible female and resistant female×susceptible male) responded alike in bioassays,with values of 0.14 and 0.11 for DML,respectively,which indicated autosomal inheritance and incomplete recessive.Chi-square analysis of growth and development of progeny from backcross(F1×Resistant strain) and self-cross of backcross suggested that resistance was controlled primarily by one major loci.
Keywords:Bt toxin  Transgenic cotton  Cotton bollworm  Resistance genetics
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