酶联免疫吸附分析法测定土壤中的毒死蜱

采用自制多克隆抗体,考察了pH值和甲醇含量对竞争反应的影响,并对反应体系进行了优化,最终建立了土壤中毒死蜱的残留检测ELISA方法。试验表明,毒死蜱对抗体竞争性结合的I50为76.8μg/L,方法检出限为5.2μg/L。土壤样品经简单前处理后,分别采用ELISA和GC测定,用ELISA方法测得土壤中毒死蜱的添加回收率为80.33%~83.36%,CV为5.28%~9.83%,最低检出限为0.005mg/kg,与GC测定结果基本相当,符合农药残留分析的要求。

Determination of Chlorpyrifos in Soil by ELISA

Using the self-made poly-clonal antibody, the affect of pH and the methanol content on the competitive reaction were investigated, and the reaction system was optimized. An indirect enzyme-linked immunosorbent assay (ELISA) for the determination of chlorpyrifos residue in soil was developed. The results showed that the chlorpyrifos concentration(I_(50))that inhibits 50% of antibody-antigen binding was 76.8 μg/L,the detection limit I_(20) was 5.2 μg/L. Soil samples were detected with ELISA and GC after simple pre-procession. The fortified recovery of ELISA were 80.33%-(83.36%,)CV were 5.28%-9.83%, the determination limit was 0.005 mg/kg. The results of the ELISA were verified by GC method.