高效氯氰菊酯对草鱼鳃和脾脏溶菌酶活性的影响

研究了暴露在高效氯氰菊酯中的草鱼(Ctenopharyngodon idella)鳃和脾脏溶菌酶(LSZ)的活性变化。高效氯氰菊酯设5个剂量组,分别为0μg/L、0.5μg/L、1.0μg/L、3.0μg/L和5.0μg/L组,每组分别于1d、5d和12d取样,测定鳃和脾脏LSZ活性。结果显示,在暴露1d、5d时,鳃LSZ活性表现为:0.5μg/L组(82.701、117.334U/g)和1.0μg/L组(103.703、128.167U/g)组显著或极显著上升(P<0.05,P<0.01),3.0μg/L组(40.045、31.357U/g)和5.0μg/L组(23.421、15.433U/g)显著或极显著下降(P<0.05,P<0.01);暴露12d时,鳃LSZ活性表现为:0.5μg/L组(38.489U/g)、1.0μg/L组(30.247U/g)、3.0μg/L组(20.694U/g)和5.0μg/L组(9.668U/g)酶活均极显著下降(P<0.01)。暴露1d时,脾脏LSZ活性表现为:0.5μg/L(68.232U/g)、1.0μg/L(90.884U/g)、3.0μg/L(116.219U/g)和5.0μg/L(60.636U/g)组均极显著上升(P<0.01);暴露5d,脾脏LSZ活性表现为:0.5μg/L(86.718U/g)、1.0μg/L(108.394U/g)和3.0μg/L(98.194U/g)组极显著上升(P<0.01),5.0μg/L(21.395U/g)组极显著下降(P<0.01);暴露12d,0.5μg/L(100.422U/g)、1.0μg/L(80.605U/g)组极显著上升(P<0.01),3.0μg/L(30.853U/g)和5.0μg/L(12.046U/g)显著或极显著下降(P<0.05,P<0.01)。表明高效氯氰菊酯对草鱼鳃和脾脏具有明显的毒害作用。

Effect of Beta-cypermethrin on Lysozyme Activity in Gill and Spleen of Grass Carp

The change on the activity of lysozyme in gill and spleen of grass carp exposed to different concentration of beta-cypermethrin was studied in this paper.The concentrations of beta-cypermethrin used in the experiment were 0 μg/L,0.5 μg/L,1.0 μg/L,3.0 μg/L and 5.0 μg/L,respectively.The activities of gill and spleen lysozyme were determined on the first,fifth and twelfth days of each group.The results showed that 0.5 μg/L and 1.0 μg/L groups of gill LSZ activities increased significantly(P<0.05 or P<0.01) while for the 3.0 μg/L and 5.0 μg/L groups decreased remarkably(P<0.05 or P<0.01) when exposed to the first day and fifth day of the samples.Gill LSZ activities of the 0.5 μg/L,1.0 μg/L,3.0 μg/L and 5.0 μg/L groups decreased remarkably(P<0.01) when exposed to the twelfth day.In the case of spleen,when exposed to the first day,the LSZ activities for 0.5 μg/L,1.0 μg/L,3.0 μg/L and 5.0 μg/L groups increased significantly(P<0.01).The LSZ activities for the 0.5 μg/L,1.0 μg/L and 3.0 μg/L groups increased remarkably(P<0.01) while the 5.0 μg/L group LSZ activities decreased significantly(P<0.01) when exposed to the fifth day.When exposed to twelfth day,LSZ activities of the 0.5 μg/L and 1.0 μg/L groups increased signi-ficantly(P<0.01).Accordingly,LSZ activities of the 3.0 μg/L and 5.0 μg/L groups decreased remarkably(P<0.05 or P<0.01).It indicates that beta-cypermethrin has significant toxic effect on the gill and spleen of grass carp.