| 农杆菌介导的绿木霉ZBS6的T-DNA转化研究 |
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| 引用本文： | 孙虎,施艳,薛保国,杨丽荣,梁慎,全鑫,刘德畅.农杆菌介导的绿木霉ZBS6的T-DNA转化研究[J].河南农业科学,2011,40(12):106-109. |
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| 作者姓名： | 孙虎 施艳 薛保国 杨丽荣 梁慎 全鑫 刘德畅 |
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| 作者单位： | 1. 河南省农业科学院植物保护研究所/河南省农作物病虫害防治重点实验室,河南郑州,450002
2. 河南农业大学植物保护学院,河南郑州,450002 |
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| 基金项目： | 国家自然科学基金青年基金项目(31000886) |
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| 摘 要： | 应用农杆菌介导的T - DNA转化技术,成功转化了生防绿木霉(Trichoderma viride)ZBS6,共获得368个转化子,转化效率为每106个分生孢子产生92个转化子.PCR和Southern blot分子鉴定以及遗传稳定性测定结果表明:转化子T - DNA插入率为90％,均能够稳定遗传,且T-DNA单拷贝插...
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| 关 键 词： | 绿木霉 T - DNA转化 农杆菌 转化子 生防作用 |
Agrobacterium-mediated T-DNA Transformation of Trichoderma viride ZBS6 |
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| SUN Hu
,
SHI Yan
,
XUE Bao-guo
,
YANG Li-rong
,
LIANG Shen
,
QUAN Xin
,
LIU De-chang.Agrobacterium-mediated T-DNA Transformation of Trichoderma viride ZBS6[J].Journal of Henan Agricultural Sciences,2011,40(12):106-109. |
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| Authors: | SUN Hu SHI Yan XUE Bao-guo YANG Li-rong LIANG Shen QUAN Xin LIU De-chang |
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| Institution: | 1(1.Institute of Plant Protection/Key Laboratory of Crop Diseases and Insect Pests Prevention in Henan,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | In this study,Trichoderma viride ZBS6 was transformed based on agrobacterium-mediated T-DNA transformation technology and 368 transformants were obtained.The transformation efficiency was 92 transformants per 106 spores.The transformants were identified using PCR and Southern blot as well as genetic stability assay.The results showed that the percentage of positive transformants which inherited stably achieved as high as 90% and among them 80% were single-copy transformants.Further indoor biocontrol assay was conducted with Gaeumannomyces gramims var.tritici as test pathogen.Two transformants,T79 and T124,which showed obviously increased antagonism,were screened.This study laid the foundation for functional genomics study of T.viride and also supplied enough materials for insights into the molecular mechanism of antagonism. |
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| Keywords: | Trichoderma viride T-DNA transformation Agrobacterium Transformants Biological control |
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