| 铁棍山药PPO的最适底物、热稳定性及褐变控制研究 |
| |
| 引用本文： | 刘莹,赵喜亭,赵月丽,鲁梦娇,成淑君,赵雪蕾,李明军,刘亚丽.铁棍山药PPO的最适底物、热稳定性及褐变控制研究[J].河南农业科学,2010(12). |
| |
| 作者姓名： | 刘莹 赵喜亭 赵月丽 鲁梦娇 成淑君 赵雪蕾 李明军 刘亚丽 |
| |
| 基金项目： | 河南师范大学博士启动基金，河南师范大学大学生创新性实验计划项目 |
| |
| 摘 要： | 研究了铁棍山药多酚氧化酶(PPO)的最适底物、热稳定性及不同抑制剂对其活性的影响。结果表明:在试验范围内,铁棍山药PPO最适底物为邻苯二酚;在较低温度(20～40℃)下其PPO能保持较稳定的活性,较高温度(40～80℃)下其活性快速下降,80℃下保温10 min,PPO活性几乎为零;抑制剂对PPO活性的抑制效果依次为:L-抗坏血酸>L-半胱氨酸盐酸盐>植酸>柠檬酸。
|
| 关 键 词： | 铁棍山药 多酚氧化酶 热稳定性 褐变 |
Studies on the Best Substrate, the Thermal Stability of Polyphenol Oxidase and the Browning Control in Dioscorea oppostia Thunb.cv.Tiegun |
| |
| LIU Ying,ZHAO Xi-ting,ZHAO Yue-li,LU Meng-jiao,CHENG Shu-jun,ZHAO Xue-lei,LI Ming-jun,LIU Ya-li.Studies on the Best Substrate, the Thermal Stability of Polyphenol Oxidase and the Browning Control in Dioscorea oppostia Thunb.cv.Tiegun[J].Journal of Henan Agricultural Sciences,2010(12). |
| |
| Authors: | LIU Ying ZHAO Xi-ting ZHAO Yue-li LU Meng-jiao CHENG Shu-jun ZHAO Xue-lei LI Ming-jun LIU Ya-li |
| |
| Abstract: | The best substrate,the thermal stability of polyphenol oxidase(PPO) from Dioscorea oppostia Thunb.cv.Tiegun and the effects of inhibitor on its activity were studied in this paper.The results showed that: The best substrate of PPO from Dioscorea oppostia Thunb.cv.Tiegun was catechol in the range of this experiment;the activity of PPO was more stable under the lower temperature(20-40℃),while,it's avtivity declined rapidly under the higher temperature(40-80℃)and was inhibited almost completely when incubated for 10min under 80℃;the order of inhibition effect from strong to weak was:L-ascorbic acid,L-cysteine hydrochloride,phytic acid,citric acid. |
| |
| Keywords: | Dioscorea oppostia Thunb cv Tiegun Polyphenol oxidase Thermal stability Browning |
| 本文献已被 万方数据 等数据库收录！ |
|
