| 细胞松弛素B对延边黄牛体细胞核移植效率的影响 |
| |
| 引用本文： | 宋君博,林涛,胡艳明,李钟淑,方南洙.细胞松弛素B对延边黄牛体细胞核移植效率的影响[J].河南农业科学,2009(4). |
| |
| 作者姓名： | 宋君博 林涛 胡艳明 李钟淑 方南洙 |
| |
| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
| |
| 基金项目： | 国家自然科学基金，振兴东北老工业基地项目，延边大学合作项目 |
| |
| 摘 要： | 探讨融合液、激活液中添加细胞松弛素B(CCB)对延边黄牛体细胞核移植效率的影响,以摸索适合延边黄牛体细胞核移植的电融合、激活条件。结果表明,融合液中添加7.5μg/mL CCB组的桑葚胚及囊胚发育率显著高于其他3组;激活液中添加5.0μg/mL的CCB组的卵裂率、桑葚胚及囊胚发育率显著高于其他3组;融合液、激活液中同时添加CCB的囊胚发育率高于单独添加组,但是差异不显著。
|
| 关 键 词： | 细胞松弛素B 体细胞核移植 融合液 激活液 延边黄牛 |
Effects of Cytochalasin B on the Efficiency of Somatic Cell Nuclear Transfer in Yanbian Yellow Cattle |
| |
| SONG Jun-bo,LIN Tao,HU Yan-ming,LI Zhong-shu,FANG Nan-zhu.Effects of Cytochalasin B on the Efficiency of Somatic Cell Nuclear Transfer in Yanbian Yellow Cattle[J].Journal of Henan Agricultural Sciences,2009(4). |
| |
| Authors: | SONG Jun-bo LIN Tao HU Yan-ming LI Zhong-shu FANG Nan-zhu |
| |
| Institution: | College of Agriculture;Yanbian University;Longjing 133400;China |
| |
| Abstract: | To investigate the best conditions of electrofusion and activation for nuclear transplantation in Yanbian yellow cattle,the cytochalasin B(CCB) was added in the medium of fusion and activation and the efficiency of somatic cell nuclear transfer was determined.The result showed that the morula rate and the rate of development into blastocyst stage of the group added with 7.5μg/mL CCB in fusion medium were significantly higher than that of other groups.The clea-vage rate,morula rate and the rate of development into blastocyst stage of the group added with 5.0 μg/mL CCB in activation medium were significantly higher than that of other groups.When the CCB was added in the fusion and activation media together,the rate of development into blastocyst stage was slightly higher than that added with CCB in fusion or activation medium alone,but no significant difference was observed. |
| |
| Keywords: | Cytochalasin B Somatic cell nuclear transfer Fusion medium Activation medium Yanbian yellow cattle |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
