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| 玫瑰黄链霉菌Men-myco-93-63中3个调控基因的检测 | |
| 引用本文： | 冀红柳,胡亚亚,沈凤英,李亚宁,刘大群.玫瑰黄链霉菌Men-myco-93-63中3个调控基因的检测[J].河南农业科学,2013,42(3). |
| 作者姓名： | 冀红柳 胡亚亚 沈凤英 李亚宁 刘大群 |
| 作者单位： | 1. 河北农业大学植物保护学院河北省植物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心,河北保定071001;邢台市园林局,河北邢台054000 2. 河北农业大学植物保护学院河北省植物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心,河北保定,071001 3. 河北北方学院,河北张家口,075000 |
| 基金项目： | 国家自然科学基金项目，国家高技术研究与发展计划(863)项目，河北省自然科学基金项目 |
| 摘 要： | 玫瑰黄链霉菌(Streptomyces roseoflavus)Men-myco-93-63及其发酵液对多种重要植物病原菌均表现出很强的抑制作用,在植物病害生物防治方面有很大的应用潜力。根据天蓝色链霉菌A3(2)调控基因tcrA、sarA和scrX序列设计引物,扩增Men-myco-93-63基因组DNA,分别获得705bp、1 472bp和473bp的片段,经测序和比对,上述片段与天蓝色链霉菌A3(2)的tcrA、sarA和scrX基因在核苷酸序列和氨基酸序列上同源性均为99%,因此,推测玫瑰黄链霉菌Men-myco-93-63中很可能含有这些调控基因。Men-myco-93-63中这3个调控基因的初步检测为玫瑰黄链霉菌功能基因的获得、菌株的遗传改良和代谢调控途径的研究提供了重要依据。 |
| 关 键 词： | 玫瑰黄链霉菌 调控基因 tcrA基因 sarA基因 scrX基因 |
Detection of tcrA and Other Regulator Genes from Streptomyces roseo flavus Men-myco-93-63 |
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| JI Hong-liu , HU Ya-ya , SHEN Feng-ying , LI Ya-ning , LIU Da-qun.Detection of tcrA and Other Regulator Genes from Streptomyces roseo flavus Men-myco-93-63[J].Journal of Henan Agricultural Sciences,2013,42(3). | |
| Authors: | JI Hong-liu HU Ya-ya SHEN Feng-ying LI Ya-ning LIU Da-qun |
| Abstract: | Streptomyces roseoflavus Men-myco-93-63 and its fermentation provide very well control effects on many important pathogenic fungi,and have shown a great deal of production and application potential in biological control of plant diseases.In this study,three fragments of 705 bp,1 472 bp and 473 bp,corresponding with the tcrA,sarA and scrX gene of Men-myco-93-63,respectively,were amplified by the primers designed based on the corresponding gene sequences of S.coelicolor A3(2).After sequencing,Blastn and Blastx analyses,the nucleotide sequences and amino acid sequences of the three fragments from Men-myco-93-63 had 99% homology separately with the tcrA,sarA and scrX gene of S.coelicolor A3(2).So,S.roseoflavus Men-myco-93-63 might contain these regulator genes.The detection will provide an important basis for gain of functional genes,performance of genetic transformation and research of metabolic pathway of S.roseoflavus. |
| Keywords: | Streptomyces roseoflavus regulator genes tcrA sarA scrX |
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