|
|||||
|
|
| 蝴蝶兰叶片诱导类原球茎初探 | |
| 引用本文： | 张玉,李艳敏,张和臣,王利民,王慧娟,张鸽香.蝴蝶兰叶片诱导类原球茎初探[J].河南农业科学,2012,41(2):126-128,135. |
| 作者姓名： | 张玉 李艳敏 张和臣 王利民 王慧娟 张鸽香 |
| 作者单位： | 1. 南京林业大学风景园林学院,江苏南京210037;河南省农业科学院园艺研究所,河南郑州450002 2. 河南省农业科学院园艺研究所,河南郑州,450002 3. 南京林业大学风景园林学院,江苏南京,210037 |
| 基金项目： | 河南省重点科技攻关项目 |
| 摘 要： | 以蝴蝶兰杂交品种H807无菌苗的叶片为材料,研究了防褐化物质(柠檬酸、抗坏血酸及活性炭)和细胞分裂素(6-BA与TDZ)对类原球茎诱导的影响。结果表明:在添加70mg/L抗坏血酸的培养基中,叶片褐化级别仅为1级,防褐化效果最佳;3mg/L的TDZ对蝴蝶兰叶片诱导类原球茎的诱导率最高,为75.0%。在以1/2MS附加70 mg/L抗坏血酸的基础上,激素组合TDZ3mg/L+NAA 1mg/L对蝴蝶兰H807类原球茎的诱导效果最佳。 |
| 关 键 词： | 蝴蝶兰 叶片 褐化 类原球茎 诱导率 |
Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis |
|
| ZHANG Yu , LI Yan-min , ZHANG He-chen , WANG Li-min , WANG Hui-juan , ZHANG Ge-xiang.Study on Protocorn-like Body Induced from the Leaves of Phalaenopsis amabilis[J].Journal of Henan Agricultural Sciences,2012,41(2):126-128,135. | |
| Authors: | ZHANG Yu LI Yan-min ZHANG He-chen WANG Li-min WANG Hui-juan ZHANG Ge-xiang |
| Institution: | 1 (1.College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China; 2.Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China) |
| Abstract: | There are serious browning and low induction rate when the protocorn-like body(PLB) was induced from leaves of Phalaenopsis amabilis.Using the grem free leaf of Phalaenopsis amabilis H807 as materials,the effects of anti-browning materials(citric acid,ascorbic acid and activated charcoal)and cytokinins(6-BA and TDZ) on the protocorn-like body induction was investigated.The results indicated that the rank of anti-browning only reached the level 1 with 70 mg/L ascorbic acid,which was best to prevent in vitro leaves from browning.The protocorn-like body induction rate reached 75.0% with 3 mg/L TDZ.On the medium with 1/2MS+70 mg/L ascorbic acid,hormone combination of 3 mg/L TDZ+1 mg/L NAA was the best for PLB induction. |
| Keywords: | Phalaenopsis amabilis leaf browning the protocorn-like body induction rate |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |