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茜草茎段组织培养灭菌方法研究
引用本文:于相丽,李勇慧,王静.茜草茎段组织培养灭菌方法研究[J].河南农业科学,2011,40(7):130-131,134.
作者姓名:于相丽  李勇慧  王静
作者单位:洛阳师范学院,河南,洛阳471022
基金项目:河南省科技厅科技攻关项目
摘    要:研究了不同灭菌剂(0.1%HgCl2、10%NaClO、10%H2O2)、不同灭菌时间、二步灭菌法对茜草不同年龄的茎段污染率和愈伤组织诱导率的影响.结果表明:75%酒精处理30 s+10%NaClO灭菌20 rain效果最好,污染率20%,愈伤组织诱导率为35%;二步灭菌法不适合于茜草茎段的灭菌;幼嫩茜草茎段愈伤组织诱...

关 键 词:茜草  灭菌方法  污染率  愈伤组织诱导率

Sterilization Conditions for Tissue Culture of Stem In Rubia cordifolia L.
YU Xiang-Li,LI Yong-hui,WANG Jing.Sterilization Conditions for Tissue Culture of Stem In Rubia cordifolia L.[J].Journal of Henan Agricultural Sciences,2011,40(7):130-131,134.
Authors:YU Xiang-Li  LI Yong-hui  WANG Jing
Institution:(College of Life Science,Luoyang Normal University,Luoyang 471022,China)
Abstract:The different age stems from Rubia cordifolia L.were used as explants to study the effects of different bactericides(0.1%HgCl2,10%NaClO,10%H2O2),different sterilization times,and two-step asepsis on pollution of explants and induction of callus.The results showed that the method of 75% alcohol 30s+10% NaClO 20min was the best and the pollution rat was 20%,induction rate was 35%;the method of two-step asepsis was suitable for stems of Rubia cordifolia L.;the young stems'induction rate of callus was the highest up to 55%.
Keywords:Rubia cordifolia L    Sterilization method  Pollution rate  Induction rate
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