| 海洋双RNA病毒VP2e基因的克隆和表达 |
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| 引用本文： | 林建国,胡瑛,王常高,蔡俊.海洋双RNA病毒VP2e基因的克隆和表达[J].河南农业科学,2009(7). |
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| 作者姓名： | 林建国 胡瑛 王常高 蔡俊 |
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| 作者单位： | 湖北工业大学,生物工程学院,工业微生物湖北省重点实验室,湖北,武汉,430068 |
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| 基金项目： | 湖北省教育厅中青年项目，湖北工业大学校基金 |
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| 摘 要： | 从海洋双RNA病毒(MABV)Y-6毒株基因组中克隆到VP2 e基因,将其连接到GST融合表达载体pGEX-6P-1,在大肠杆菌中表达,并对其表达条件进行了优化。通过温度和诱导物浓度优化试验,经SDS-PAGE和Western-Blot检测表明,在温度为37℃、IPTG浓度为0.1mmol/L时,表达的融合蛋白含量最高,占细菌总蛋白的28.6%。
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| 关 键 词： | 海洋双RNA病毒 VP2e基因 克隆 表达 |
Cloning and Expression of the VP2e Gene of Marine Birnavirus in E.coli |
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| LIN Jian-guo,HU Ying,WANG Chang-gao,CAI Jun.Cloning and Expression of the VP2e Gene of Marine Birnavirus in E.coli[J].Journal of Henan Agricultural Sciences,2009(7). |
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| Authors: | LIN Jian-guo HU Ying WANG Chang-gao CAI Jun |
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| Abstract: | The VP2e gene was amplified from the genome of marine birnavirus (MABV) by RT-PCR. Then, the PCR products were cloned into the GST fusion expression vector pGEX-6P-1 and expressed in E.coli. The conditions including expression temperature and IPTG-induction concentration were optimized. The results of SDS-PAGE and Western-blot showed that the expression level of the fusion protein occupies 28.6% of the total bacterial proteins under the optimal conditions (37℃, 0.1mmol/L IPTG). |
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| Keywords: | MABV VP2e gene Clone Expression |
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