| 正交试验优化余甘子愈伤组织诱导培养基研究 |
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| 引用本文： | 黄勇,张铁,沈尚东.正交试验优化余甘子愈伤组织诱导培养基研究[J].甘肃农业科技,2018(3):49-51. |
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| 作者姓名： | 黄勇 张铁 沈尚东 |
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| 作者单位： | 文山学院; |
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| 摘 要： | 以余甘子无菌幼苗作为材料,通过正交试验研究影响余甘子愈伤组织诱导的因素,以完善余甘子组织培养体系。结果表明:以茎或叶作为外植体,在MS+1.0 mg/L 2,4-D+0.5 mg/L 6-BA培养基中,愈伤组织诱导率达87.9%以上,愈伤组织生长良好。
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| 关 键 词： | 余甘子 愈伤组织 组织培养 离体快繁 正交试验 |
Study on Optimizing the Inducing Medium for Calli of Phyllanthus emblica through Orthogonal Test |
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| HUANG Yong,ZHANG Tie,SHEN Shangdong.Study on Optimizing the Inducing Medium for Calli of Phyllanthus emblica through Orthogonal Test[J].Gansu Agricultural Science and Technology,2018(3):49-51. |
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| Authors: | HUANG Yong ZHANG Tie SHEN Shangdong |
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| Abstract: | Using asepsis seedling of Phyllanthus emblica as material, the factors that affect the callus induced were studied, in order to improve the system of tissue culture for Phyllanthus emblica through orthogonal experiment. The results show that when using stems or leaves as explants, inducement rate of calli was above 87.9% and calli grew well in the medium of MS+1.0 mg/L 2,4-D +0.5 mg/L 6-BA. |
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