| 马铃薯茎尖超低温保存研究 |
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| 引用本文： | 王永林.马铃薯茎尖超低温保存研究[J].甘肃农业科技,2006(10):3-5. |
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| 作者姓名： | 王永林 |
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| 作者单位： | 甘肃省兰州市农业科学研究所,甘肃,兰州,730000 |
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| 基金项目： | 感谢甘肃农业大学生命科学技术学院司怀军副教授在研究过程中给予的帮助. |
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| 摘 要： | 以马铃薯栽培品种甘农薯1号的试管苗为材料,进行了茎尖超低温保存技术的研究,结果表明,预培养3～4 d,用玻璃化溶液PVS2室温下处理20～30 min,直接投入液氮中冷冻保存,然后接种在MS添加2.50 mg/L KT、1.25 mg/L IAA和1.25 mg/L 6-BA的培养基上,经过恢复培养,茎尖成活率可达42.8%.
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| 关 键 词： | 马铃薯 茎尖 超低温保存 |
| 文章编号： | 1001-1463（2006）10-0003-03 |
| 收稿时间： | 2006-08-08 |
| 修稿时间： | 2006年8月8日 |
Study on Cryopreservation of Potato Shoot-tips |
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| WANG Yong-lin.Study on Cryopreservation of Potato Shoot-tips[J].Gansu Agricultural Science and Technology,2006(10):3-5. |
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| Authors: | WANG Yong-lin |
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| Institution: | Lanzhou Institute of Agricultural Sciences, Lanzhou Gansu 730000, China |
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| Abstract: | Shoot tips of plantlet in vitro of potato cultivar Gannongshu 1 as donors, cryopreservation of shoot tips was studied in the experiment. The excised shoot-tips, were precultured 3-4 d and treated by vitrification solution PVS2 for 20- 30 rain under room temperature, plunged into fluid nitrogen and stored for a certain time then transferred onto MS medium supplemented with 2. 5 mg/L KT, 1.25 mg/L IAA and 1.25 mg/L 6-BA, survival rate of shoot tips of potato could reached 42.8%. |
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| Keywords: | Potato Shoot-tip Cryopreservation |
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