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山茱萸多糖提取工艺优化及马钱苷含量测定
引用本文:常玲彬.山茱萸多糖提取工艺优化及马钱苷含量测定[J].甘肃农业科技,2011(11):26-29.
作者姓名:常玲彬
作者单位:[1]甘肃农业大学食品工程学院,甘肃兰州730070;[2]甘肃省天水市农业科学研究所,甘肃天水741001
摘    要:采用L(934)正交设计试验,对山茱萸浸提液中山茱萸多糖的酶水解法提取工艺进行了优化研究,并对浸提液的中有效成分马钱苷含量进行了HPLC法分析。结果表明,山茱萸多糖浸提的最佳工艺为:液料比1∶5,浸提时间4 h,浸提温度80℃,果胶酶添加量0.55 g/L。用HPLC法测定出的山茱萸浸提液中马钱苷平均含量为0.512 ...

关 键 词:山茱萸  多糖  浸提液  提取工艺  HPLC法  马钱苷含量

Optimization of Extraction Process of Polysaccharides of Cornus and Determination of the Contents of loganin
CHANG Ling-bin.Optimization of Extraction Process of Polysaccharides of Cornus and Determination of the Contents of loganin[J].Gansu Agricultural Science and Technology,2011(11):26-29.
Authors:CHANG Ling-bin
Institution:CHANG Ling-bin1,2(1.College of Food Engineering,Gansu Agricultural University,Lanzhou Gansu 730070,China,2.Tianshui Institute of Agricultural Sciences,Tianshui Gansu 741001,China)
Abstract:The Extraction Process of Zhongshan Cornus polysaccharides in leach liquors was studied through enzyme hydrolysis,and the extract the active ingredient of loganin were analysis by HPLC.The results showed that the best process of cornel polysaccharide extraction was the ratio of solvent to material was 1∶5;the optimum extraction time was 6 h,extraction temperacture was 80 ℃,the optimum addition of pectin enzyme was 0.55 g/L.The average content of loganin in leach liquors was 0.512 g/L by HPLC method.
Keywords:Cornus  Polysaccharide  Leaching solution  Extraction process  HPLC method  The content of loganin  
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