| 花粉管通道法转基因棉花后代筛选鉴定 |
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| 引用本文： | 李忠旺,厚毅清,石有太,罗俊杰,陈玉梁.花粉管通道法转基因棉花后代筛选鉴定[J].甘肃农业科技,2012(4):14-16. |
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| 作者姓名： | 李忠旺 厚毅清 石有太 罗俊杰 陈玉梁 |
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| 作者单位： | 甘肃省农业科学院生物技术研究所,甘肃兰州,730070 |
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| 基金项目： | 国家转基因生物新品种培育重大专项“西北干旱区转基因抗旱彩色棉新种质创制研究” |
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| 摘 要： | 通过对棕色棉BC05叶片进行Kan浓度梯度涂抹实验,确定了Kan涂抹筛选的最佳浓度为3.0 g/L,最佳观察时间为涂抹第5天。对Kan抗性植株进行PCR鉴定表明,Kan涂抹筛选可基本准确的鉴别出转基因材料与非转基因材料,但目标基因是否成功整合还必须进行PCR鉴定,田间卡那霉素筛选结合PCR分子鉴定是转基因棉花后代大规模群体筛选的有效方法。
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| 关 键 词： | 花粉管通道法 转基因 棉花 后代筛选 鉴定 |
Screening and Identification of Transgenic Offspring Cotton by Pollen-tube Pathway Method |
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| Institution: | LI Zhong-wang,HOU Yi-qing,SHI You-tai,LUO Jun-jie,CHEN Yu-ling(Institute of Biotechnology,Gansu Academy of Agriculture Sciences,Lanzhou Gansu 730070,China) |
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| Abstract: | The smear test of Kan concentration gradient was conducted in brown cotton BC05 leaves,then the best screening concentration of 3.0 g/L and the best observation time of fifth day were obtained.Through the PCR identification in Kan-resistant plants,the results showed that the method was able to identify whether plant is genetically modified material or non-GM material.But whether the target gene was successfully integrated was remain to be identified by PCR.Therefore,the kanamycin screening combining with PCR molecular identification is an effective method to screened large groups of genetically modified cotton offspring. |
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| Keywords: | Pollen-tube pathway Transgenic Cotton Offspring screen Identification |
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